Stabilization of RAD51 nucleoprotein filaments by the C-terminal region of BRCA2 - PubMed (original) (raw)
Comparative Study
doi: 10.1038/nsmb1245. Epub 2007 May 21.
Affiliations
- PMID: 17515904
- DOI: 10.1038/nsmb1245
Comparative Study
Stabilization of RAD51 nucleoprotein filaments by the C-terminal region of BRCA2
Fumiko Esashi et al. Nat Struct Mol Biol. 2007 Jun.
Abstract
The human breast cancer susceptibility gene BRCA2 is required for the regulation of RAD51-mediated homologous recombinational repair. BRCA2 interacts with RAD51 monomers, as well as nucleoprotein filaments, primarily though the conserved BRC motifs. The unrelated C-terminal region of BRCA2 also interacts with RAD51. Here we show that the BRCA2 C terminus interacts directly with RAD51 filaments, but not monomers, by binding an interface created by two adjacent RAD51 protomers. These interactions stabilize filaments so that they cannot be dissociated by association with BRC repeats. Interaction of the BRCA2 C terminus with the RAD51 filament causes a large movement of the flexible RAD51 N-terminal domain that is important in regulating filament dynamics. We suggest that interactions of the BRCA2 C-terminal region with RAD51 may facilitate efficient nucleation of RAD51 multimers on DNA and thereby stimulate recombination-mediated repair.
Comment in
- RAD51, BRCA2 and DNA repair: a partial resolution.
Lord CJ, Ashworth A. Lord CJ, et al. Nat Struct Mol Biol. 2007 Jun;14(6):461-2. doi: 10.1038/nsmb0607-461. Nat Struct Mol Biol. 2007. PMID: 17549079 No abstract available.
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- 2002:445/BCN_/Breast Cancer Now/United Kingdom
- 2004NOV03/BCN_/Breast Cancer Now/United Kingdom
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