Mediating effect of dopamine D3 receptors on Jak2 and GABAAalpha1 expression in mouse brains induced by cocaine - PubMed (original) (raw)

. 2007 May 20;120(10):910-4.

Affiliations

PMID: 17543182

Mediating effect of dopamine D3 receptors on Jak2 and GABAAalpha1 expression in mouse brains induced by cocaine

Nu-yun Liu et al. Chin Med J (Engl). 2007.

Abstract

Background: Cocaine addiction may involve complex neuroadaptations, including many changes of genes expression. Dopamine D3 receptors play an important role in cocaine addiction; however, its role in cocaine induced gene expression change is poorly understood. To identify the changes in gene expression induced by repeated cocaine exposure through D3 dopamine receptors, we compared the expression of four molecules: Janus kinase 2 (Jak2), g-aminobutanoic acid receptor subunit alpha 1 (GABAAalpha1), glutamate receptor AMPA3 alpha 3 (GluR 3) and stromal cell derived factor 1 (SDF1). These four have been implicated in mediating the actions of cocaine in the nucleus accumbens (NAc) and caudoputamen (CPu) in mice after acute and repeated cocaine exposure.

Methods: For the acute and repeated injections, the mice were divided into four groups: 30 mg/kg cocaine, nafadotride 0.5 mg/kg + cocaine 30 mg/kg, nafadotride 0.5 mg/kg, and saline as the basal group. The expression of Jak2, GABAAalpha1, GluR 3 and SDF1 were assayed by Western blot, quantitative real-time RT-PCR and immunohistochemistry.

Results: Twenty-four hours after seven consecutive days of repeated cocaine exposure, the expression of GABAAalpha1 decreased in cocaine group compared with basal line and further decreased in the cocaine + nafadotride group and remained at basal level in the nafadotride group. Similarly, the Jak2 expression decreased in cocaine group compared with base line. However, the levels of Jak2 increased in cocaine + nafadotride group compared with cocaine group, while remained at basal level in nafadotride group.

Conclusions: GABAAalpha1 and Jak2 may be involved in chronic cocaine induced neuroadaptations. D3 dopamine receptors play an important role in the expression of these genes.

PubMed Disclaimer

Cited by

SYVN1, an ERAD E3 Ubiquitin Ligase, Is Involved in GABAAα1 Degradation Associated with Methamphetamine-Induced Conditioned Place Preference.
Jiao DL, Chen Y, Liu Y, Ju YY, Long JD, Du J, Yu CX, Wang YJ, Zhao M, Liu JG. Jiao DL, et al. Front Mol Neurosci. 2017 Oct 5;10:313. doi: 10.3389/fnmol.2017.00313. eCollection 2017. Front Mol Neurosci. 2017. PMID: 29051727 Free PMC article.
The neural rejuvenation hypothesis of cocaine addiction.
Dong Y, Nestler EJ. Dong Y, et al. Trends Pharmacol Sci. 2014 Aug;35(8):374-83. doi: 10.1016/j.tips.2014.05.005. Epub 2014 Jun 20. Trends Pharmacol Sci. 2014. PMID: 24958329 Free PMC article. Review.
Activation of Dopamine D1 Receptors Regulates Dendritic Morphogenesis Through Rac1 and RhoA in Prefrontal Cortex Neurons.
Li J, Gu J, Wang B, Xie M, Huang L, Liu Y, Zhang L, Xue J, Guo F, Zhang L, Zhang L. Li J, et al. Mol Neurobiol. 2015;51(3):1024-37. doi: 10.1007/s12035-014-8762-1. Epub 2014 Jun 12. Mol Neurobiol. 2015. PMID: 24915967 Free PMC article.
JAK2 regulates Nav1.6 channel function via FGF14Y158 phosphorylation.
Wadsworth PA, Singh AK, Nguyen N, Dvorak NM, Tapia CM, Russell WK, Stephan C, Laezza F. Wadsworth PA, et al. Biochim Biophys Acta Mol Cell Res. 2020 Oct;1867(10):118786. doi: 10.1016/j.bbamcr.2020.118786. Epub 2020 Jun 26. Biochim Biophys Acta Mol Cell Res. 2020. PMID: 32599005 Free PMC article.

Mediating effect of dopamine D3 receptors on Jak2 and GABAAalpha1 expression in mouse brains induced by cocaine - PubMed (original) (raw)