Requirements for T lymphocyte migration in explanted lymph nodes - PubMed (original) (raw)

. 2007 Jun 15;178(12):7747-55.

doi: 10.4049/jimmunol.178.12.7747.

L Isabel Cárdenas-Navia, Charles C Caldwell, Troy J Plumb, Caius G Radu, Paulo N Rocha, Tuere Wilder, Jonathan S Bromberg, Bruce N Cronstein, Michail Sitkovsky, Mark W Dewhirst, Michael L Dustin

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Requirements for T lymphocyte migration in explanted lymph nodes

Julie H Huang et al. J Immunol. 2007.

Abstract

Although the requirements for T lymphocyte homing to lymph nodes (LNs) are well studied, much less is known about the requirements for T lymphocyte locomotion within LNs. Imaging of murine T lymphocyte migration in explanted LNs using two-photon laser-scanning fluorescence microscopy provides an opportunity to systematically study these requirements. We have developed a closed system for imaging an intact LN with controlled temperature, oxygenation, and perfusion rate. Naive T lymphocyte locomotion in the deep paracortex of the LN required a perfusion rate of >13 microm/s and a partial pressure of O(2) (pO(2)) of >7.4%. Naive T lymphocyte locomotion in the subcapsular region was 38% slower and had higher turning angles and arrest coefficients than naive T lymphocytes in the deep paracortex. T lymphocyte activation decreased the requirement for pO(2), but also decreased the speed of locomotion in the deep paracortex. Although CCR7(-/-) naive T cells displayed a small reduction in locomotion, systemic treatment with pertussis toxin reduced naive T lymphocyte speed by 59%, indicating a contribution of Galpha(i)-mediated signaling, but involvement of other G protein-coupled receptors besides CCR7. Receptor knockouts or pharmacological inhibition in the adenosine, PG/lipoxygenase, lysophosphatidylcholine, and sphingosine-1-phosphate pathways did not individually alter naive T cell migration. These data implicate pO(2), tissue architecture, and G-protein coupled receptor signaling in regulation of naive T lymphocyte migration in explanted LNs.

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