Structural basis for the recognition between the regulatory particles Nas6 and Rpt3 of the yeast 26S proteasome - PubMed (original) (raw)

. 2007 Aug 3;359(3):503-9.

doi: 10.1016/j.bbrc.2007.05.138. Epub 2007 May 29.

Affiliations

• PMID: 17555716
• DOI: 10.1016/j.bbrc.2007.05.138

Structural basis for the recognition between the regulatory particles Nas6 and Rpt3 of the yeast 26S proteasome

Yoshihiro Nakamura et al. Biochem Biophys Res Commun. 2007.

Abstract

The 26S proteasome-dependent protein degradation is an evolutionarily conserved process. The mammalian oncoprotein gankyrin, which associates with S6 of the proteasome, facilitates the degradation of pRb, and thus possibly acts as a bridging factor between the proteasome and its substrates. However, the mechanism of the proteasome-dependent protein degradation in yeast is poorly understood. Here, we report the tertiary structure of the complex between Nas6 and a C-terminal domain of Rpt3, which are the yeast orthologues of gankyrin and S6, respectively. The concave region of Nas6 bound to the alpha-helical domain of Rpt3. The stable interaction between Nas6 and Rpt3 was mediated by intermolecular interactions composed of complementary charged patches. The recognition of Rpt3 by Nas6 in the crystal suggests that Nas6 is indeed a subunit of the 26S proteasome. These results provide a structural basis for the association between Nas6 and the heterohexameric ATPase ring of the proteasome through Rpt3.

PubMed Disclaimer

Similar articles

• Structure of the oncoprotein gankyrin in complex with S6 ATPase of the 26S proteasome.
  Nakamura Y, Nakano K, Umehara T, Kimura M, Hayashizaki Y, Tanaka A, Horikoshi M, Padmanabhan B, Yokoyama S. Nakamura Y, et al. Structure. 2007 Feb;15(2):179-89. doi: 10.1016/j.str.2006.11.015. Structure. 2007. PMID: 17292836
• Multiple proteasome-interacting proteins assist the assembly of the yeast 19S regulatory particle.
  Saeki Y, Toh-E A, Kudo T, Kawamura H, Tanaka K. Saeki Y, et al. Cell. 2009 May 29;137(5):900-13. doi: 10.1016/j.cell.2009.05.005. Epub 2009 May 14. Cell. 2009. PMID: 19446323
• Purification, crystallization and preliminary X-ray diffraction analysis of the non-ATPase subunit Nas6 in complex with the ATPase subunit Rpt3 of the 26S proteasome from Saccharomyces cerevisiae.
  Nakamura Y, Umehara T, Tanaka A, Horikoshi M, Padmanabhan B, Yokoyama S. Nakamura Y, et al. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Mar 1;63(Pt 3):190-2. doi: 10.1107/S1744309107004848. Epub 2007 Feb 10. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007. PMID: 17329811 Free PMC article.
• Chaperone-driven proteasome assembly.
  Rosenzweig R, Glickman MH. Rosenzweig R, et al. Biochem Soc Trans. 2008 Oct;36(Pt 5):807-12. doi: 10.1042/BST0360807. Biochem Soc Trans. 2008. PMID: 18793141 Review.
• Assembly and function of the proteasome.
  Saeki Y, Tanaka K. Saeki Y, et al. Methods Mol Biol. 2012;832:315-37. doi: 10.1007/978-1-61779-474-2_22. Methods Mol Biol. 2012. PMID: 22350895 Review.

Cited by

• Proteasome Complexes and Their Heterogeneity in Colorectal, Breast and Pancreatic Cancers.
  Zagirova D, Autenried R, Nelson ME, Rezvani K. Zagirova D, et al. J Cancer. 2021 Mar 5;12(9):2472-2487. doi: 10.7150/jca.52414. eCollection 2021. J Cancer. 2021. PMID: 33854609 Free PMC article.
• Stable incorporation of ATPase subunits into 19 S regulatory particle of human proteasome requires nucleotide binding and C-terminal tails.
  Lee SH, Moon JH, Yoon SK, Yoon JB. Lee SH, et al. J Biol Chem. 2012 Mar 16;287(12):9269-79. doi: 10.1074/jbc.M111.316208. Epub 2012 Jan 24. J Biol Chem. 2012. PMID: 22275368 Free PMC article.
• Cell biology: The proteasome assembly line.
  Madura K. Madura K. Nature. 2009 Jun 11;459(7248):787-8. doi: 10.1038/459787a. Nature. 2009. PMID: 19516331 Free PMC article.
• The ubiquitin proteasome system in Caenorhabditis elegans and its regulation.
  Papaevgeniou N, Chondrogianni N. Papaevgeniou N, et al. Redox Biol. 2014 Jan 18;2:333-47. doi: 10.1016/j.redox.2014.01.007. eCollection 2014. Redox Biol. 2014. PMID: 24563851 Free PMC article. Review.
• An atomic model AAA-ATPase/20S core particle sub-complex of the 26S proteasome.
  Förster F, Lasker K, Beck F, Nickell S, Sali A, Baumeister W. Förster F, et al. Biochem Biophys Res Commun. 2009 Oct 16;388(2):228-33. doi: 10.1016/j.bbrc.2009.07.145. Epub 2009 Aug 3. Biochem Biophys Res Commun. 2009. PMID: 19653995 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Molecular Biology Databases

  • BioCyc
  • Saccharomyces Genome Database