Synteny and chromosome evolution in the lepidoptera: evidence from mapping in Heliconius melpomene - PubMed (original) (raw)

Synteny and chromosome evolution in the lepidoptera: evidence from mapping in Heliconius melpomene

Elizabeth G Pringle et al. Genetics. 2007 Sep.

Abstract

The extent of conservation of synteny and gene order in the Lepidoptera has been investigated previously only by comparing a small subset of linkage groups between the moth Bombyx mori and the butterfly Heliconius melpomene. Here we report the mapping of 64 additional conserved genes in H. melpomene, which contributed 47 markers to a comparative framework of 72 orthologous loci spanning all 21 H. melpomene chromosomes and 27 of the 28 B. mori chromosomes. Comparison of the maps revealed conserved synteny across all chromosomes for the 72 loci, as well as evidence for six cases of chromosome fusion in the Heliconius lineage that contributed to the derived 21-chromosome karyotype. Comparisons of gene order on these fused chromosomes revealed two instances of colinearity between H. melpomene and B. mori, but also one instance of likely chromosomal rearrangement. B. mori is the first lepidopteran species to have its genome sequenced, and the finding that there is conserved synteny and gene order among Lepidoptera indicates that the genomic tools developed in B. mori will be broadly useful in other species.

PubMed Disclaimer

Figures

F<sc>igure</sc> 1.—

Figure 1.—

Phylogeny used to estimate time of divergence between B. mori and H. melpomene. The dashed line indicates the approximate age of divergence between these lineages (∼103 MY). Arrows indicate constrained nodes (see

materials and methods

). Numbers in circles indicate the haploid number of chromosomes for B. mori, H. melpomene, and basal taxa.

F<sc>igure</sc> 2.—

Figure 2.—

Linkage maps of putatively fused chromosomes in H. melpomene with comparison to maps of conserved markers in B. mori (A–F, corresponding to the six different putatively fused chromosomes in H. melpomene). Note the difference in scale between the maps. The lack of position bars for RpL13, ptc, and _Ef1_α in B. mori LG5 indicates that these markers were mapped using BAC–FISH instead of recombination linkage mapping (see Y

asukochi

et al. 2006). The lack of position bars for RpS16 in B. mori LG14 and H. melpomene LG13 indicates the lack of recombination mapping in B. mori and recombination mapping in a different brood (brood 44 as opposed to brood 33; see

materials and methods

) in H. melpomene.

F<sc>igure</sc> 2.—

Figure 2.—

Linkage maps of putatively fused chromosomes in H. melpomene with comparison to maps of conserved markers in B. mori (A–F, corresponding to the six different putatively fused chromosomes in H. melpomene). Note the difference in scale between the maps. The lack of position bars for RpL13, ptc, and _Ef1_α in B. mori LG5 indicates that these markers were mapped using BAC–FISH instead of recombination linkage mapping (see Y

asukochi

et al. 2006). The lack of position bars for RpS16 in B. mori LG14 and H. melpomene LG13 indicates the lack of recombination mapping in B. mori and recombination mapping in a different brood (brood 44 as opposed to brood 33; see

materials and methods

) in H. melpomene.

Similar articles

Cited by

References

    1. Behura, S. K., 2006. Molecular marker systems in insects: current trends and future avenues. Mol. Ecol. 15: 3087–3113. - PubMed
    1. Beldade, P., S. Rudd, J. D. Gruber and A. D. Long, 2006. A wing expressed sequence tag resource for Bicyclus anynana butterflies, an evo-devo model. BMC Genomics 7: 130. - PMC - PubMed
    1. Blaxter, M., 2003. Comparative genomics: two worms are better than one. Nature 426: 395–396. - PubMed
    1. Brown, G. R., E. E. Kadel, D. L. Bassoni, K. L. Kiehne, B. Temesgen et al., 2001. Anchored reference loci in loblolly pine (Pinus taeda L.) for integrating pine genomics. Genetics 159: 799–809. - PMC - PubMed
    1. Brown, K. S., T. C. Emmel, P. J. Eliazar and E. Suomalainen, 1992. Evolutionary patterns in chromosome-numbers in neotropical Lepidoptera.1. Chromosomes of the Heliconiini (family Nymphalidae, subfamily Nymphalinae). Hereditas 117: 109–125. - PubMed

Publication types

MeSH terms

LinkOut - more resources