LambdaN-GFP: an RNA reporter system for live-cell imaging - PubMed (original) (raw)
doi: 10.1038/nmeth1065. Epub 2007 Jul 1.
Affiliations
- PMID: 17603490
- DOI: 10.1038/nmeth1065
LambdaN-GFP: an RNA reporter system for live-cell imaging
Nathalie Daigle et al. Nat Methods. 2007 Aug.
Abstract
We describe a GFP-based RNA reporter system (lambdaN-GFP) to visualize RNA molecules in live mammalian cells. It consists of GFP fused to an arginine-rich peptide derived from the phage lambda N protein, lambdaN22, which binds a unique minimal RNA motif and can be used to tag any RNA molecule. LambdaN-GFP uses a small and easy to engineer RNA tag, reducing the likelihood of perturbing the function of the tagged RNA molecule.
Similar articles
- Direct detection of RNA transcription by FRET imaging using fluorescent protein probe.
Endoh T, Mie M, Kobatake E. Endoh T, et al. J Biotechnol. 2008 Feb 29;133(4):413-7. doi: 10.1016/j.jbiotec.2007.11.005. Epub 2007 Nov 19. J Biotechnol. 2008. PMID: 18160117 - A GFP-based assay for monitoring post-transcriptional regulation of ARE-mRNA turnover.
Benjamin D, Colombi M, Stoecklin G, Moroni C. Benjamin D, et al. Mol Biosyst. 2006 Nov;2(11):561-7. doi: 10.1039/b609448a. Epub 2006 Sep 27. Mol Biosyst. 2006. PMID: 17216037 - Use of GFP to analyze morphology, connectivity, and function of cells in the central nervous system.
Harvey AR, Ehlert E, de Wit J, Drummond ES, Pollett MA, Ruitenberg M, Plant GW, Verhaagen J, Levelt CN. Harvey AR, et al. Methods Mol Biol. 2009;515:63-95. doi: 10.1007/978-1-59745-559-6_5. Methods Mol Biol. 2009. PMID: 19378117 - Using fluorescent proteins to study mRNA trafficking in living cells.
Querido E, Chartrand P. Querido E, et al. Methods Cell Biol. 2008;85:273-92. doi: 10.1016/S0091-679X(08)85012-1. Methods Cell Biol. 2008. PMID: 18155467 Review. - The uses of green fluorescent protein in prokaryotes.
Valdivia RH, Cormack BP, Falkow S. Valdivia RH, et al. Methods Biochem Anal. 2006;47:163-78. Methods Biochem Anal. 2006. PMID: 16335713 Review. No abstract available.
Cited by
- Biogenesis and dynamics of the coronavirus replicative structures.
Hagemeijer MC, Rottier PJ, de Haan CA. Hagemeijer MC, et al. Viruses. 2012 Nov 21;4(11):3245-69. doi: 10.3390/v4113245. Viruses. 2012. PMID: 23202524 Free PMC article. Review. - MONITTR allows real-time imaging of transcription and endogenous proteins in C. elegans.
Liu X, Chang Z, Sun P, Cao B, Wang Y, Fang J, Pei Y, Chen B, Zou W. Liu X, et al. J Cell Biol. 2025 Jan 6;224(1):e202403198. doi: 10.1083/jcb.202403198. Epub 2024 Oct 14. J Cell Biol. 2025. PMID: 39400293 - Long-term dual-color tracking of genomic loci by modified sgRNAs of the CRISPR/Cas9 system.
Shao S, Zhang W, Hu H, Xue B, Qin J, Sun C, Sun Y, Wei W, Sun Y. Shao S, et al. Nucleic Acids Res. 2016 May 19;44(9):e86. doi: 10.1093/nar/gkw066. Epub 2016 Feb 4. Nucleic Acids Res. 2016. PMID: 26850639 Free PMC article. - Using high-throughput barcode sequencing to efficiently map connectomes.
Peikon ID, Kebschull JM, Vagin VV, Ravens DI, Sun YC, Brouzes E, Corrêa IR Jr, Bressan D, Zador AM. Peikon ID, et al. Nucleic Acids Res. 2017 Jul 7;45(12):e115. doi: 10.1093/nar/gkx292. Nucleic Acids Res. 2017. PMID: 28449067 Free PMC article. - Fluorescence fluctuation spectroscopy enables quantitative imaging of single mRNAs in living cells.
Wu B, Chao JA, Singer RH. Wu B, et al. Biophys J. 2012 Jun 20;102(12):2936-44. doi: 10.1016/j.bpj.2012.05.017. Epub 2012 Jun 19. Biophys J. 2012. PMID: 22735544 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials