Constitutive expression of the urokinase plasminogen activator gene in murine RAW264 macrophages involves distal and 5' non-coding sequences that are conserved between mouse and pig - PubMed (original) (raw)
Constitutive expression of the urokinase plasminogen activator gene in murine RAW264 macrophages involves distal and 5' non-coding sequences that are conserved between mouse and pig
A I Cassady et al. Nucleic Acids Res. 1991.
Free PMC article
Abstract
The 5' flanking regions of the mouse and pig urokinase plasminogen activator (uPA) genes were sequenced and sequence homology interrupted by repeat elements was found to extend to -4.6kb in pig and -6.6kb in mouse. A transient transfection procedure was devised for the murine macrophage cell line RAW264. Pig uPA promoter-CAT constructs were more active than mouse constructs in this assay. This contrast may involve sequence differences within 100 bp of the transcription start site. The selective deletion of distal regions of the promoter (greater than 2.6 kb upstream), and of a conserved element, 5'-AGGAGGAAATGAGG-TCA-3' around -2 kb greatly reduced the activity of reporter constructs in RAW264 cells. Electrophoretic mobility shift assays using the latter sequence identified a single nuclear protein complex. This element has been referred to as PEA3/AP1-like, but the complex did not comigrate with either AP1 or known proteins that bind polypurines (including the macrophage-specific factor PU-1) and was not competed by AP1 or polypurine oligonucleotides. uPA promoters contain multiple AP1 and AP2-like DNA sequences, which were recognised by nuclear proteins expressed constitutively in RAW264 cells. They also contain multiple binding sites for NF kappa B but activated NF kappa B was not expressed in RAW264 cells. The conserved, transcribed 5' non-coding sequences were also required for maximal gene expression. Hence, the uPA promoter contains multiple weak cis-acting elements distributed over 7.0 kb 5' to the translation start site.
Similar articles
- Synergistic transcriptional activation of the mouse urokinase plasminogen activator (uPA) gene and of its enhancer activator protein 1 (AP1) site by cAMP and retinoic acid.
Mira-Y-Lopez R, Jaramillo S, Jing Y. Mira-Y-Lopez R, et al. Biochem J. 1998 May 1;331 ( Pt 3)(Pt 3):909-16. doi: 10.1042/bj3310909. Biochem J. 1998. PMID: 9560322 Free PMC article. - Cooperation of two PEA3/AP1 sites in uPA gene induction by TPA and FGF-2.
D'Orazio D, Besser D, Marksitzer R, Kunz C, Hume DA, Kiefer B, Nagamine Y. D'Orazio D, et al. Gene. 1997 Nov 12;201(1-2):179-87. doi: 10.1016/s0378-1119(97)00445-9. Gene. 1997. PMID: 9409785 - Ras regulation of urokinase-type plasminogen activator.
Lengyel E, Ried S, Heiss MM, Jäger C, Schmitt M, Allgayer H. Lengyel E, et al. Methods Enzymol. 2001;333:105-16. doi: 10.1016/s0076-6879(01)33049-5. Methods Enzymol. 2001. PMID: 11400328 Review. No abstract available.
Cited by
- Purification and cDNA cloning of a transcription factor which functionally cooperates within a cAMP regulatory unit in the porcine uPA gene.
Menoud PA, Matthies R, Hofsteenge J, Nagamine Y. Menoud PA, et al. Nucleic Acids Res. 1993 Apr 25;21(8):1845-52. doi: 10.1093/nar/21.8.1845. Nucleic Acids Res. 1993. PMID: 8388098 Free PMC article. - Macrophage nitric oxide synthase gene: two upstream regions mediate induction by interferon gamma and lipopolysaccharide.
Lowenstein CJ, Alley EW, Raval P, Snowman AM, Snyder SH, Russell SW, Murphy WJ. Lowenstein CJ, et al. Proc Natl Acad Sci U S A. 1993 Oct 15;90(20):9730-4. doi: 10.1073/pnas.90.20.9730. Proc Natl Acad Sci U S A. 1993. PMID: 7692452 Free PMC article. - Cytoskeletal reorganization and TPA differently modify AP-1 to induce the urokinase-type plasminogen activator gene in LLC-PK1 cells.
Lee JS, von der Ahe D, Kiefer B, Nagamine Y. Lee JS, et al. Nucleic Acids Res. 1993 Jul 25;21(15):3365-72. doi: 10.1093/nar/21.15.3365. Nucleic Acids Res. 1993. PMID: 8346015 Free PMC article. - Alterations in differentiation and behavior of monocytic phagocytes in transgenic mice that express dominant suppressors of ras signaling.
Jin DI, Jameson SB, Reddy MA, Schenkman D, Ostrowski MC. Jin DI, et al. Mol Cell Biol. 1995 Feb;15(2):693-703. doi: 10.1128/MCB.15.2.693. Mol Cell Biol. 1995. PMID: 7823938 Free PMC article.
References
- Cell. 1991 Mar 8;64(5):961-9 - PubMed
- Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 - PubMed
- Biochemistry. 1987 Dec 15;26(25):8270-9 - PubMed
- J Exp Med. 1986 Dec 1;164(6):2113-8 - PubMed
- Nucleic Acids Res. 1988 Aug 11;16(15):7527-44 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
Miscellaneous