Ascorbic acid protects against endogenous oxidative DNA damage in human sperm - PubMed (original) (raw)
Ascorbic acid protects against endogenous oxidative DNA damage in human sperm
C G Fraga et al. Proc Natl Acad Sci U S A. 1991.
Abstract
Damage to the DNA of germ cells can lead to mutation, which may result in birth defects, genetic diseases, and cancer. The very high endogenous rate of oxidative DNA damage and the importance of dietary ascorbic acid (AA) in preventing this damage has prompted an examination of these factors in human sperm DNA. The oxidized nucleoside 8-hydroxy-2'-deoxyguanosine (8-oxo-7,8-dihydro-2'-deoxyguanosine; oxo8dG), 1 of approximately 20 major products of oxidative damage to DNA, was measured in DNA isolated from human sperm provided by healthy subjects and compared to the seminal fluid AA levels. This relationship was studied in two groups. In a group of 24 free-living individuals 20-50 years old high levels of oxo8dG were correlated with low seminal plasma AA. The endogenous level of oxo8dG in this group was 13 fmol per microgram of DNA or approximately 25,000 adducts per sperm cell. The second group of individuals was maintained on a controlled diet that varied only in AA content. When dietary AA was decreased from 250 to 5 mg/day, the seminal fluid AA decreased by half and the level of oxo8dG in sperm DNA increased 91%. Repletion of dietary AA for 28 days (from 5 mg/day to 250 or 60 mg/day) caused a doubling in seminal fluid AA and reduced oxo8dG by 36%. These results indicate that dietary AA protects human sperm from endogenous oxidative DNA damage that could affect sperm quality and increase risk of genetic defects, particularly in populations with low AA such as smokers.
Similar articles
- Smoking and low antioxidant levels increase oxidative damage to sperm DNA.
Fraga CG, Motchnik PA, Wyrobek AJ, Rempel DM, Ames BN. Fraga CG, et al. Mutat Res. 1996 Apr 13;351(2):199-203. doi: 10.1016/0027-5107(95)00251-0. Mutat Res. 1996. PMID: 8622715 - The associations among semen quality, oxidative DNA damage in human spermatozoa and concentrations of cadmium, lead and selenium in seminal plasma.
Xu DX, Shen HM, Zhu QX, Chua L, Wang QN, Chia SE, Ong CN. Xu DX, et al. Mutat Res. 2003 Jan 10;534(1-2):155-63. doi: 10.1016/s1383-5718(02)00274-7. Mutat Res. 2003. PMID: 12504764 - Oxidative DNA damage estimated by oxo8dG in the liver of guinea-pigs supplemented with graded dietary doses of ascorbic acid and alpha-tocopherol.
Cadenas S, Barja G, Poulsen HE, Loft S. Cadenas S, et al. Carcinogenesis. 1997 Dec;18(12):2373-7. doi: 10.1093/carcin/18.12.2373. Carcinogenesis. 1997. PMID: 9450484 - Detection of oxidative DNA damage in human sperm and its association with sperm function and male infertility.
Shen H, Ong C. Shen H, et al. Free Radic Biol Med. 2000 Feb 15;28(4):529-36. doi: 10.1016/s0891-5849(99)00234-8. Free Radic Biol Med. 2000. PMID: 10719234 Review. - Endogenous oxidative damage of mtDNA.
Beckman KB, Ames BN. Beckman KB, et al. Mutat Res. 1999 Mar 8;424(1-2):51-8. doi: 10.1016/s0027-5107(99)00007-x. Mutat Res. 1999. PMID: 10064849 Review.
Cited by
- Enhanced Efficacy of Direct-Acting Antivirals in Hepatitis C Patients by Coadministration of Black Cumin and Ascorbate as Antioxidant Adjuvants.
Ahmed S, Zahoor A, Ibrahim M, Younus M, Nawaz S, Naseer R, Akram Q, Deng CL, Ojha SC. Ahmed S, et al. Oxid Med Cell Longev. 2020 Jun 3;2020:7087921. doi: 10.1155/2020/7087921. eCollection 2020. Oxid Med Cell Longev. 2020. PMID: 32566096 Free PMC article. Clinical Trial. - Reactive oxygen species and oocyte aging: role of superoxide, hydrogen peroxide, and hypochlorous acid.
Goud AP, Goud PT, Diamond MP, Gonik B, Abu-Soud HM. Goud AP, et al. Free Radic Biol Med. 2008 Apr 1;44(7):1295-304. doi: 10.1016/j.freeradbiomed.2007.11.014. Epub 2007 Dec 8. Free Radic Biol Med. 2008. PMID: 18177745 Free PMC article. - Nutritional modifications in male infertility: a systematic review covering 2 decades.
Giahi L, Mohammadmoradi S, Javidan A, Sadeghi MR. Giahi L, et al. Nutr Rev. 2016 Feb;74(2):118-30. doi: 10.1093/nutrit/nuv059. Epub 2015 Dec 24. Nutr Rev. 2016. PMID: 26705308 Free PMC article. Review. - Effects of split applications of nitrogen fertilizers on the Cd level and nutritional quality of Chinese cabbage.
Fan SK, Zhu J, Tian WH, Guan MY, Fang XZ, Jin CW. Fan SK, et al. J Zhejiang Univ Sci B. 2017 Oct.;18(10):897-905. doi: 10.1631/jzus.B1600272. J Zhejiang Univ Sci B. 2017. PMID: 28990380 Free PMC article. - Etiologies of sperm oxidative stress.
Sabeti P, Pourmasumi S, Rahiminia T, Akyash F, Talebi AR. Sabeti P, et al. Int J Reprod Biomed. 2016 Apr;14(4):231-40. Int J Reprod Biomed. 2016. PMID: 27351024 Free PMC article. Review.
References
- Ann N Y Acad Sci. 1987;498:312-23 - PubMed
- Adv Hum Genet. 1975;5:223-318 - PubMed
- Proc Natl Acad Sci U S A. 1990 Jun;87(12):4533-7 - PubMed
- Proc Natl Acad Sci U S A. 1991 May 1;88(9):3633-6 - PubMed
- Am J Epidemiol. 1991 Jan 15;133(2):123-32 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical