Telomere protection by mammalian Pot1 requires interaction with Tpp1 - PubMed (original) (raw)

doi: 10.1038/nsmb1270. Epub 2007 Jul 15.

Affiliations

PMID: 17632522
DOI: 10.1038/nsmb1270

Telomere protection by mammalian Pot1 requires interaction with Tpp1

Dirk Hockemeyer et al. Nat Struct Mol Biol. 2007 Aug.

Erratum in

Nat Struct Mol Biol. 2009 May;16(5):572

Abstract

The shelterin complex at mammalian telomeres contains the single-stranded DNA-binding protein Pot1, which regulates telomere length and protects chromosome ends. Pot1 binds Tpp1, the shelterin component that connects Pot1 to the duplex telomeric DNA-binding proteins Trf1 and Trf2. Control of telomere length requires that Pot1 binds Tpp1 as well as the single-stranded telomeric DNA, but it is not known whether the protective function of Pot1 depends on Tpp1. Alternatively, Pot1 might function similarly to the Pot1-like proteins of budding and fission yeast, which have no known Tpp1-like connection to the duplex telomeric DNA. Using mutant mouse cells with diminished Tpp1 levels, RNA interference directed to mouse Tpp1 and Pot1, and complementation of mouse Pot1 knockout cells with human and mouse Pot1 variants, we show here that Tpp1 is required for the protective function of mammalian Pot1 proteins.

PubMed Disclaimer

Cited by

Similarities and differences between "uncapped" telomeres and DNA double-strand breaks.
Dewar JM, Lydall D. Dewar JM, et al. Chromosoma. 2012 Apr;121(2):117-30. doi: 10.1007/s00412-011-0357-2. Epub 2011 Dec 28. Chromosoma. 2012. PMID: 22203190 Review.
Structural biology of telomeres and telomerase.
Smith EM, Pendlebury DF, Nandakumar J. Smith EM, et al. Cell Mol Life Sci. 2020 Jan;77(1):61-79. doi: 10.1007/s00018-019-03369-x. Epub 2019 Nov 14. Cell Mol Life Sci. 2020. PMID: 31728577 Free PMC article. Review.
TPP1 is required for TERT recruitment, telomere elongation during nuclear reprogramming, and normal skin development in mice.
Tejera AM, Stagno d'Alcontres M, Thanasoula M, Marion RM, Martinez P, Liao C, Flores JM, Tarsounas M, Blasco MA. Tejera AM, et al. Dev Cell. 2010 May 18;18(5):775-89. doi: 10.1016/j.devcel.2010.03.011. Dev Cell. 2010. PMID: 20493811 Free PMC article.
Roles of the checkpoint sensor clamp Rad9-Rad1-Hus1 (911)-complex and the clamp loaders Rad17-RFC and Ctf18-RFC in Schizosaccharomyces pombe telomere maintenance.
Khair L, Chang YT, Subramanian L, Russell P, Nakamura TM. Khair L, et al. Cell Cycle. 2010 Jun 1;9(11):2237-48. doi: 10.4161/cc.9.11.11920. Epub 2010 Jun 1. Cell Cycle. 2010. PMID: 20505337 Free PMC article.
Multiple POT1-TPP1 proteins coat and compact long telomeric single-stranded DNA.
Taylor DJ, Podell ER, Taatjes DJ, Cech TR. Taylor DJ, et al. J Mol Biol. 2011 Jul 1;410(1):10-7. doi: 10.1016/j.jmb.2011.04.049. Epub 2011 May 9. J Mol Biol. 2011. PMID: 21596049 Free PMC article.

Telomere protection by mammalian Pot1 requires interaction with Tpp1 - PubMed (original) (raw)