The CDP-ethanolamine pathway and phosphatidylserine decarboxylation generate different phosphatidylethanolamine molecular species - PubMed (original) (raw)
. 2007 Sep 28;282(39):28362-28372.
doi: 10.1074/jbc.M703786200. Epub 2007 Aug 2.
Affiliations
- PMID: 17673461
- DOI: 10.1074/jbc.M703786200
Free article
The CDP-ethanolamine pathway and phosphatidylserine decarboxylation generate different phosphatidylethanolamine molecular species
Onno B Bleijerveld et al. J Biol Chem. 2007.
Free article
Abstract
In mammalian cells, phosphatidylethanolamine (PtdEtn) is mainly synthesized via the CDP-ethanolamine (Kennedy) pathway and by decarboxylation of phosphatidylserine (PtdSer). However, the extent to which these two pathways contribute to overall PtdEtn synthesis both quantitatively and qualitatively is still not clear. To assess their contributions, PtdEtn species synthesized by the two routes were labeled with pathway-specific stable isotope precursors, d(3)-serine and d(4)-ethanolamine, and analyzed by high performance liquid chromatography-mass spectrometry. The major conclusions from this study are that (i) in both McA-RH7777 and Chinese hamster ovary K1 cells, the CDP-ethanolamine pathway was favored over PtdSer decarboxylation, and (ii) both pathways for PtdEtn synthesis are able to produce all diacyl-PtdEtn species, but most of these species were preferentially made by one pathway. For example, the CDP-ethanolamine pathway preferentially synthesized phospholipids with mono- or di-unsaturated fatty acids on the sn-2 position (e.g. (16:0-18:2)PtdEtn and (18:1-18:2)PtdEtn), whereas PtdSer decarboxylation generated species with mainly polyunsaturated fatty acids on the sn-2 position (e.g. (18:0-20:4)PtdEtn and (18:0-20:5)PtdEtn in McArdle and (18: 0-20:4)PtdEtn and (18:0-22:6)PtdEtn in Chinese hamster ovary K1 cells). (iii) The main PtdEtn species newly synthesized from the Kennedy pathway in the microsomal fraction appeared to equilibrate rapidly between the endoplasmic reticulum and mitochondria. (iv) Newly synthesized PtdEtn species preferably formed in the mitochondria, which is at least in part due to the substrate specificity of the phosphatidylserine decarboxylase, seemed to be retained in this organelle. Our data suggest a potentially essential role of the PtdSer decarboxylation pathway in mitochondrial functioning.
Similar articles
- Contribution of different pathways to the supply of phosphatidylethanolamine and phosphatidylcholine to mitochondrial membranes of the yeast Saccharomyces cerevisiae.
Bürgermeister M, Birner-Grünberger R, Nebauer R, Daum G. Bürgermeister M, et al. Biochim Biophys Acta. 2004 Nov 8;1686(1-2):161-8. doi: 10.1016/j.bbalip.2004.09.007. Biochim Biophys Acta. 2004. PMID: 15522832 - Formation and function of phosphatidylserine and phosphatidylethanolamine in mammalian cells.
Vance JE, Tasseva G. Vance JE, et al. Biochim Biophys Acta. 2013 Mar;1831(3):543-54. doi: 10.1016/j.bbalip.2012.08.016. Epub 2012 Aug 29. Biochim Biophys Acta. 2013. PMID: 22960354 Review. - Biosynthetic regulation and intracellular transport of phosphatidylserine in mammalian cells.
Kuge O, Nishijima M. Kuge O, et al. J Biochem. 2003 Apr;133(4):397-403. doi: 10.1093/jb/mvg052. J Biochem. 2003. PMID: 12761285 Review.
Cited by
- Biochemistry and Diseases Related to the Interconversion of Phosphatidylcholine, Phosphatidylethanolamine, and Phosphatidylserine.
Korbecki J, Bosiacki M, Kupnicka P, Barczak K, Ziętek P, Chlubek D, Baranowska-Bosiacka I. Korbecki J, et al. Int J Mol Sci. 2024 Oct 6;25(19):10745. doi: 10.3390/ijms251910745. Int J Mol Sci. 2024. PMID: 39409074 Free PMC article. Review. - Innovations in Antifungal Drug Discovery among Cell Envelope Synthesis Enzymes through Structural Insights.
Zhou Y, Reynolds TB. Zhou Y, et al. J Fungi (Basel). 2024 Feb 22;10(3):171. doi: 10.3390/jof10030171. J Fungi (Basel). 2024. PMID: 38535180 Free PMC article. Review. - Phospholipid isotope tracing reveals β-catenin-driven suppression of phosphatidylcholine metabolism in hepatocellular carcinoma.
VanSant-Webb C, Low HK, Kuramoto J, Stanley CE, Qiang H, Su A, Ross AN, Cooper CG, Cox JE, Summers SA, Evason KJ, Ducker GS. VanSant-Webb C, et al. bioRxiv [Preprint]. 2023 Oct 16:2023.10.12.562134. doi: 10.1101/2023.10.12.562134. bioRxiv. 2023. PMID: 37904922 Free PMC article. Updated. Preprint. - Modulation of Dietary Choline Uptake in a Mouse Model of Acid Sphingomyelinase Deficiency.
Gaudioso Á, Moreno-Huguet P, Casas J, Schuchman EH, Ledesma MD. Gaudioso Á, et al. Int J Mol Sci. 2023 Jun 5;24(11):9756. doi: 10.3390/ijms24119756. Int J Mol Sci. 2023. PMID: 37298714 Free PMC article. - Plasma Lipidomic Subclasses and Risk of Hypertension in Middle-Aged and Elderly Chinese.
Niu Z, Wu Q, Luo Y, Wang D, Zheng H, Wu Y, Yang X, Zeng R, Sun L, Lin X. Niu Z, et al. Phenomics. 2022 Jun 14;2(5):283-294. doi: 10.1007/s43657-022-00057-y. eCollection 2022 Oct. Phenomics. 2022. PMID: 36939788 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources