Proteomic analysis of rabbit tear fluid: Defensin levels after an experimental corneal wound are correlated to wound closure - PubMed (original) (raw)
Comparative Study
. 2007 Sep;7(17):3194-206.
doi: 10.1002/pmic.200700137.
Affiliations
- PMID: 17676663
- DOI: 10.1002/pmic.200700137
Comparative Study
Proteomic analysis of rabbit tear fluid: Defensin levels after an experimental corneal wound are correlated to wound closure
Lei Zhou et al. Proteomics. 2007 Sep.
Abstract
The cornea is the major refracting optical element of the eye and therefore critical for forming a retinal image. The exposed surface of the eye is protected from pathogens by the innate immune system whose components include defensins, naturally occurring peptides with antimicrobial properties, and the physical barrier formed by the outer epithelial layer of the cornea. The proteomic approach has revealed that tear levels of defensins are correlated with the course of healing of an experimental corneal wound. Tears were collected from New Zealand White rabbits prior to (day 0) and daily for 5 days (days 1-5) following a standard unilateral 6 mm diameter corneal epithelial abrasion. Tear protein profiles obtained from wounded and contra-lateral control eyes were compared using SELDI ProteinChip technology. Peptides and proteins of interest were purified by RP-HPLC and characterized by nanoESI-MS/MS. Mass spectra of tears on post-wound day 1, revealed 13 peaks whose level decreased and five that increased. During wound healing the tear protein profile correlated with wound closure. An important finding was that the levels of rabbit defensins (NP-1 and NP-2), which were elevated after wounding returned to normal levels by the time the corneal abrasion healed. Relative quantification of NP-2 in tear fluid prior to (day 0) and after corneal wounding (days 1- 3) was determined using iTRAQ technology. A corneal wound eliminates the barrier function of innate immunity and puts the cornea at risk from microbial attack until the epithelial cells restore the surface barrier. The increased availability of defensins in the tears during healing suggests that these peptides could protect the cornea from microbial attack during a period of increased vulnerability.
Similar articles
- Analysis of rabbit tear proteins by high-pressure liquid chromatography/electrospray ionization mass spectrometry.
Zhou L, Beuerman RW, Barathi A, Tan D. Zhou L, et al. Rapid Commun Mass Spectrom. 2003;17(5):401-12. doi: 10.1002/rcm.925. Rapid Commun Mass Spectrom. 2003. PMID: 12590388 - Nerve growth factor and corneal wound healing in dogs.
Woo HM, Bentley E, Campbell SF, Marfurt CF, Murphy CJ. Woo HM, et al. Exp Eye Res. 2005 May;80(5):633-42. doi: 10.1016/j.exer.2004.11.013. Epub 2005 Jan 4. Exp Eye Res. 2005. PMID: 15862170 - Elevation of human alpha-defensins and S100 calcium-binding proteins A8 and A9 in tear fluid of patients with pterygium.
Zhou L, Beuerman RW, Ang LP, Chan CM, Li SF, Chew FT, Tan DT. Zhou L, et al. Invest Ophthalmol Vis Sci. 2009 May;50(5):2077-86. doi: 10.1167/iovs.08-2604. Epub 2009 Jan 24. Invest Ophthalmol Vis Sci. 2009. PMID: 19168894 - Proteinases of the cornea and preocular tear film.
Ollivier FJ, Gilger BC, Barrie KP, Kallberg ME, Plummer CE, O'Reilly S, Gelatt KN, Brooks DE. Ollivier FJ, et al. Vet Ophthalmol. 2007 Jul-Aug;10(4):199-206. doi: 10.1111/j.1463-5224.2007.00546.x. Vet Ophthalmol. 2007. PMID: 17565550 Review. - Structural and functional studies of defensin-inspired peptides.
Clarke DJ, Campopiano DJ. Clarke DJ, et al. Biochem Soc Trans. 2006 Apr;34(Pt 2):251-6. doi: 10.1042/BST20060251. Biochem Soc Trans. 2006. PMID: 16545087 Review.
Cited by
- A Comparison of the Intrarectal and Intramuscular Effects of a Dexmedetomidine, Ketamine and Midazolam Mixture on Tear Production in Cats: A Randomized Controlled Trial.
Paolini A, Vignoli M, Bernabò N, Bianchi A, Tamburro R, Pincelli MC, Del Signore F, De Bonis A, Rosto M, Collivignarelli F, Distefano C, Cerasoli I. Paolini A, et al. Animals (Basel). 2023 Dec 31;14(1):145. doi: 10.3390/ani14010145. Animals (Basel). 2023. PMID: 38200876 Free PMC article. - Differential protein expression in human corneal endothelial cells cultured from young and older donors.
Zhu C, Rawe I, Joyce NC. Zhu C, et al. Mol Vis. 2008;14:1805-14. Epub 2008 Sep 30. Mol Vis. 2008. PMID: 18852868 Free PMC article. - Morphological and proteomic analysis of biofilms from the Antarctic archaeon, Halorubrum lacusprofundi.
Liao Y, Williams TJ, Ye J, Charlesworth J, Burns BP, Poljak A, Raftery MJ, Cavicchioli R. Liao Y, et al. Sci Rep. 2016 Nov 22;6:37454. doi: 10.1038/srep37454. Sci Rep. 2016. PMID: 27874045 Free PMC article. - Antimicrobial compounds in tears.
McDermott AM. McDermott AM. Exp Eye Res. 2013 Dec;117:53-61. doi: 10.1016/j.exer.2013.07.014. Epub 2013 Jul 20. Exp Eye Res. 2013. PMID: 23880529 Free PMC article. Review. - Contributions of ocular surface components to matrix-metalloproteinases (MMP)-2 and MMP-9 in feline tears following corneal epithelial wounding.
Petznick A, Madigan MC, Garrett Q, Sweeney DF, Evans MD. Petznick A, et al. PLoS One. 2013 Aug 19;8(8):e71948. doi: 10.1371/journal.pone.0071948. eCollection 2013. PLoS One. 2013. PMID: 23977185 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
Miscellaneous