Localization of vacuolar transport receptors and cargo proteins in the Golgi apparatus of developing Arabidopsis embryos - PubMed (original) (raw)
. 2007 Oct;8(10):1452-64.
doi: 10.1111/j.1600-0854.2007.00625.x. Epub 2007 Aug 13.
Affiliations
- PMID: 17696967
- DOI: 10.1111/j.1600-0854.2007.00625.x
Free article
Localization of vacuolar transport receptors and cargo proteins in the Golgi apparatus of developing Arabidopsis embryos
Giselbert Hinz et al. Traffic. 2007 Oct.
Free article
Abstract
Using immunogold electron microscopy, we have investigated the relative distribution of two types of vacuolar sorting receptors (VSR) and two different types of lumenal cargo proteins, which are potential ligands for these receptors in the secretory pathway of developing Arabidopsis embryos. Interestingly, both cargo proteins are deposited in the protein storage vacuole, which is the only vacuole present during the bent-cotyledon stage of embryo development. Cruciferin and aleurain do not share the same pattern of distribution in the Golgi apparatus. Cruciferin is mainly detected in the cis and medial cisternae, especially at the rims where storage proteins aggregate into dense vesicles (DVs). Aleurain is found throughout the Golgi stack, particularly in the trans cisternae and trans Golgi network where clathrin-coated vesicles (CCVs) are formed. Nevertheless, aleurain was detected in both DV and CCV. VSR-At1, a VSR that recognizes N-terminal vacuolar sorting determinants (VSDs) of the NPIR type, localizes mainly to the trans Golgi and is hardly detectable in DV. Receptor homology-transmembrane-RING H2 domain (RMR), a VSR that recognizes C-terminal VSDs, has a distribution that is very similar to that of cruciferin and is found in DV. Our results do not support a role for VSR-At1 in storage protein sorting, instead RMR proteins because of their distribution similar to that of cruciferin in the Golgi apparatus and their presence in DV are more likely candidates. Aleurain, which has an NPIR motif and seems to be primarily sorted via VSR-At1 into CCV, also possesses putative hydrophobic sorting determinants at its C-terminus that could allow the additional incorporation of this protein into DV.
Similar articles
- Transport of ricin and 2S albumin precursors to the storage vacuoles of Ricinus communis endosperm involves the Golgi and VSR-like receptors.
Jolliffe NA, Brown JC, Neumann U, Vicré M, Bachi A, Hawes C, Ceriotti A, Roberts LM, Frigerio L. Jolliffe NA, et al. Plant J. 2004 Sep;39(6):821-33. doi: 10.1111/j.1365-313X.2004.02167.x. Plant J. 2004. PMID: 15341626 - Vacuolar storage proteins are sorted in the cis-cisternae of the pea cotyledon Golgi apparatus.
Hillmer S, Movafeghi A, Robinson DG, Hinz G. Hillmer S, et al. J Cell Biol. 2001 Jan 8;152(1):41-50. doi: 10.1083/jcb.152.1.41. J Cell Biol. 2001. PMID: 11149919 Free PMC article. - Pathways for protein transport to seed storage vacuoles.
Jolliffe NA, Craddock CP, Frigerio L. Jolliffe NA, et al. Biochem Soc Trans. 2005 Nov;33(Pt 5):1016-8. doi: 10.1042/BST20051016. Biochem Soc Trans. 2005. PMID: 16246035 Review. - Endosomal functions in plants.
Otegui MS, Spitzer C. Otegui MS, et al. Traffic. 2008 Sep;9(10):1589-98. doi: 10.1111/j.1600-0854.2008.00787.x. Epub 2008 Jul 9. Traffic. 2008. PMID: 18627577 Review.
Cited by
- Receptor-mediated transport of vacuolar proteins: a critical analysis and a new model.
Robinson DG, Pimpl P. Robinson DG, et al. Protoplasma. 2014 Jan;251(1):247-64. doi: 10.1007/s00709-013-0542-7. Epub 2013 Sep 10. Protoplasma. 2014. PMID: 24019013 - Membrane traffic and fusion at post-Golgi compartments.
Park M, Jürgens G. Park M, et al. Front Plant Sci. 2012 Jan 4;2:111. doi: 10.3389/fpls.2011.00111. eCollection 2011. Front Plant Sci. 2012. PMID: 22645561 Free PMC article. - Mechanisms and concepts paving the way towards a complete transport cycle of plant vacuolar sorting receptors.
De Marcos Lousa C, Gershlick DC, Denecke J. De Marcos Lousa C, et al. Plant Cell. 2012 May;24(5):1714-32. doi: 10.1105/tpc.112.095679. Epub 2012 May 8. Plant Cell. 2012. PMID: 22570446 Free PMC article. Review. - Delivery of prolamins to the protein storage vacuole in maize aleurone cells.
Reyes FC, Chung T, Holding D, Jung R, Vierstra R, Otegui MS. Reyes FC, et al. Plant Cell. 2011 Feb;23(2):769-84. doi: 10.1105/tpc.110.082156. Epub 2011 Feb 22. Plant Cell. 2011. PMID: 21343414 Free PMC article. - Journey to the cell surface--the central role of the trans-Golgi network in plants.
Gendre D, Jonsson K, Boutté Y, Bhalerao RP. Gendre D, et al. Protoplasma. 2015 Mar;252(2):385-98. doi: 10.1007/s00709-014-0693-1. Epub 2014 Sep 4. Protoplasma. 2015. PMID: 25187082 Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials