Vacuolar segregation to the bud of Saccharomyces cerevisiae: an analysis of morphology and timing in the cell cycle - PubMed (original) (raw)
Comparative Study
. 1991 Oct;137(10):2447-54.
doi: 10.1099/00221287-137-10-2447.
Affiliations
- PMID: 1770360
- DOI: 10.1099/00221287-137-10-2447
Comparative Study
Vacuolar segregation to the bud of Saccharomyces cerevisiae: an analysis of morphology and timing in the cell cycle
D S Gomes de Mesquita et al. J Gen Microbiol. 1991 Oct.
Abstract
Vacuoles of Saccharomyces cerevisiae were visualized by phase-contrast microscopy. Visualization was enhanced by adding polyvinylpyrrolidone. Vacuolar segregation during the cell cycle was analysed in 42 individual cells of strain X2180 by time-lapse photomicrography. Within 15 min of bud emergence, more than 80% of the cells contained a vacuolar segregation structure in the form of either a tubule or an alignment of vesicles. The structure emerged from one point of the mother vacuole, then elongated and moved into the bud in a few minutes. The vacuolar segregation structure disappeared, usually within 20 min, before nuclear migration, leaving a separate vacuole in the bud. To test the generality of this observation several strains were grown in the presence of the vacuolar vital dye fluorescein isothiocyanate. The bud size was used to measure progress in the cell cycle. All strains formed vacuolar segregation structures in cells with small buds, although with variations in duration and timing in the cell cycle. In the presence of nocodazole vacuolar segregation occurred normally, thus, microtubules seem not to be essential in this process.
Similar articles
- Multiple methods of visualizing the yeast vacuole permit evaluation of its morphology and inheritance during the cell cycle.
Weisman LS, Bacallao R, Wickner W. Weisman LS, et al. J Cell Biol. 1987 Oct;105(4):1539-47. doi: 10.1083/jcb.105.4.1539. J Cell Biol. 1987. PMID: 2444598 Free PMC article. - Vacuole segregation in the Saccharomyces cerevisiae vac2-1 mutant: structural and biochemical quantification of the segregation defect and formation of new vacuoles.
Gomes De Mesquita DS, Shaw J, Grimbergen JA, Buys MA, Dewi L, Woldringh CL. Gomes De Mesquita DS, et al. Yeast. 1997 Sep 15;13(11):999-1008. doi: 10.1002/(SICI)1097-0061(19970915)13:11<999::AID-YEA151>3.0.CO;2-0. Yeast. 1997. PMID: 9290204 - Mutants of Saccharomyces cerevisiae that block intervacuole vesicular traffic and vacuole division and segregation.
Weisman LS, Emr SD, Wickner WT. Weisman LS, et al. Proc Natl Acad Sci U S A. 1990 Feb;87(3):1076-80. doi: 10.1073/pnas.87.3.1076. Proc Natl Acad Sci U S A. 1990. PMID: 1689059 Free PMC article. - Structure and function of the yeast vacuole and its role in autophagy.
Thumm M. Thumm M. Microsc Res Tech. 2000 Dec 15;51(6):563-72. doi: 10.1002/1097-0029(20001215)51:6<563::AID-JEMT6>3.0.CO;2-8. Microsc Res Tech. 2000. PMID: 11169858 Review. - Chapter 3. New insights into plant vacuolar structure and dynamics.
Oda Y, Higaki T, Hasezawa S, Kutsuna N. Oda Y, et al. Int Rev Cell Mol Biol. 2009;277:103-35. doi: 10.1016/S1937-6448(09)77003-0. Int Rev Cell Mol Biol. 2009. PMID: 19766968 Review.
Cited by
- A heterodimer of thioredoxin and I(B)2 cooperates with Sec18p (NSF) to promote yeast vacuole inheritance.
Xu Z, Mayer A, Muller E, Wickner W. Xu Z, et al. J Cell Biol. 1997 Jan 27;136(2):299-306. doi: 10.1083/jcb.136.2.299. J Cell Biol. 1997. PMID: 9015301 Free PMC article. - Candida albicans VAC8 is required for vacuolar inheritance and normal hyphal branching.
Barelle CJ, Richard ML, Gaillardin C, Gow NA, Brown AJ. Barelle CJ, et al. Eukaryot Cell. 2006 Feb;5(2):359-67. doi: 10.1128/EC.5.2.359-367.2006. Eukaryot Cell. 2006. PMID: 16467476 Free PMC article. - A truncated form of the Pho80 cyclin of Saccharomyces cerevisiae induces expression of a small cytosolic factor which inhibits vacuole inheritance.
Nicolson T, Conradt B, Wickner W. Nicolson T, et al. J Bacteriol. 1996 Jul;178(14):4047-51. doi: 10.1128/jb.178.14.4047-4051.1996. J Bacteriol. 1996. PMID: 8763930 Free PMC article. - Thioredoxin is required for vacuole inheritance in Saccharomyces cerevisiae.
Xu Z, Wickner W. Xu Z, et al. J Cell Biol. 1996 Mar;132(5):787-94. doi: 10.1083/jcb.132.5.787. J Cell Biol. 1996. PMID: 8603912 Free PMC article. - Determination of four biochemically distinct, sequential stages during vacuole inheritance in vitro.
Conradt B, Haas A, Wickner W. Conradt B, et al. J Cell Biol. 1994 Jul;126(1):99-110. doi: 10.1083/jcb.126.1.99. J Cell Biol. 1994. PMID: 8027190 Free PMC article.