The GluCre-ROSA26EYFP mouse: a new model for easy identification of living pancreatic alpha-cells - PubMed (original) (raw)
. 2007 Sep 4;581(22):4235-40.
doi: 10.1016/j.febslet.2007.07.068. Epub 2007 Aug 7.
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- PMID: 17706201
- DOI: 10.1016/j.febslet.2007.07.068
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The GluCre-ROSA26EYFP mouse: a new model for easy identification of living pancreatic alpha-cells
Nicolas Quoix et al. FEBS Lett. 2007.
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Abstract
The control of glucagon secretion by pancreatic alpha-cells is poorly understood, largely because of the difficulty to recognize living alpha-cells. We describe a new mouse model, referred to as GluCre-ROSA26EYFP (or GYY), allowing easy alpha-cell identification because of specific expression of EYFP. GYY mice displayed normal glycemic control during a fasting/refeeding test or intraperitoneal insulin injection. Glucagon secretion by isolated islets was normally inhibited by glucose and stimulated by adrenaline. [Ca(2+)](c) responses to arginine, adrenaline, diazoxide and tolbutamide, were similar in GYY and control mice. Hence, this new mouse model is a reliable and powerful tool to specifically study alpha-cells.
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