Fatty acid chain-elongation in perfused rat heart: synthesis of stearoylcarnitine from perfused palmitate - PubMed (original) (raw)

Fatty acid chain-elongation in perfused rat heart: synthesis of stearoylcarnitine from perfused palmitate

Janos Kerner et al. FEBS Lett. 2007.

Abstract

Rat hearts perfused for up to 60 min in the working mode with palmitate, but not with glucose, resulted in substantial formation of palmitoylcarnitine and stearoylcarnitine. To test whether lipolysis of endogenous lipids was responsible for the increased stearoylcarnitine content or whether some of the perfused palmitate underwent chain elongation, hearts were perfused with hexadecanoic-16,16,16-d(3) acid (M+3). The pentafluorophenacyl ester of deuterium labeled stearoylcarnitine had an M+3 (639.4 m/z) compared to the unlabeled M+0 (636.3 m/z) consistent with a direct chain elongation of the perfused palmitate. Furthermore, the near equal isotope enrichment of palmitoyl- (90.2+/-5.8%) and stearoylcarnitine (78.0+/-7.1%) suggest that both palmitoyl- and stearoyl-CoA have ready access to mitochondrial carnitine palmitoyltransferase and that most of the stearoylcarnitine is derived from the perfused palmitate.

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Figure 1

Figure 1

HPLC separation and mass spectrometric analysis of long-chain acylcarnitines extracted from rat hearts perfused for 60 minutes with 16,16,16-_d_3 palmitate (M+3) (1A) and unlabeled palmitate (M+0) (1B). Peak labeling: myristoylcarnitine (1); palmitoylcarnitine (2); heptadecanoylcarnitine - internal standard (3); stearoylcarnitine (4). The inserts in 1A and 1B show the mass isotopomer distribution of unlabeled (M+0) and deuterium labeled (M+3) pentaflourophenacyl esters of myristoylcarnitine (580.2; 583.3), palmitoylcarnitine (608.3; 611.3), and stearoylcarnitine (636.3; 639.4), respectively.

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References

    1. Hagve TA, Sprecher H. Metabolism of long-chain polyunsaturated fatty acids in isolated cardiac myocytes. Biochim Biophys Acta. 1989;1001:338–344. - PubMed
    1. Hamilton C, Saggerson ED. Malonyl-CoA metabolism in cardiac myocytes. Biochem J. 2000;350:61–67. - PMC - PubMed
    1. Jimenez Lopez JA, Bordoni A, Lorenzini A, Rossi CA, Biagi PL, Hrelia S. Linoleic acid metabolism in primary cultures of adult rat cardiomyocytes is impaired by aging. Biochem Biophys Res Commun. 1997;237:142–145. - PubMed
    1. Ford DA, Han X, Horner CC, Gross RW. Accumulation of unsaturated acylcarnitine molecular species during acute myocardial ischemia: metabolic compartmentalization of products of fatty acyl chain elongation in the acylcarnitine pool. Biochemistry. 1996;35:7903–7909. - PubMed
    1. Kudo N, Barr AJ, Barr RL, Desaii S, Lopaschuk GD. High rates of fatty acid oxidation during reperfusion of ischemic hearts are associated with a decrease in malonyl-CoA levels due to an increase in 5′-AMP-activated protein kinase inhibition of acetyl-CoA carboxylase. J Biol Chem. 1995;270:17513–1752. - PubMed

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