Gene expression profiling identifies Hes6 as a transcriptional target of ATOH1 in cochlear hair cells - PubMed (original) (raw)

. 2007 Oct 2;581(24):4651-6.

doi: 10.1016/j.febslet.2007.08.059. Epub 2007 Sep 4.

Affiliations

• PMID: 17826772
• DOI: 10.1016/j.febslet.2007.08.059

Free article

Gene expression profiling identifies Hes6 as a transcriptional target of ATOH1 in cochlear hair cells

Deborah Scheffer et al. FEBS Lett. 2007.

Free article

Abstract

ATOH1 is a basic Helix-Loop-Helix transcription factor crucial for hair cell (HC) differentiation in the inner ear. In order to identify ATOH1 target genes, we performed a genome-wide expression profiling analysis in cells expressing ATOH1 under the control of a tetracycline-off system and found that HES6 expression is induced by ATOH1. We performed in situ hybridisation and showed that the rise and fall of Hes6 expression closely follow that of Atoh1 in cochlear HC. Moreover, electrophoretic mobility shift assays and luciferase assays show that ATOH1 activates HES6 transcription through binding to three clustered E boxes of its promoter.

PubMed Disclaimer

Similar articles

• Basic helix-loop-helix gene Hes6 delineates the sensory hair cell lineage in the inner ear.
  Qian D, Radde-Gallwitz K, Kelly M, Tyrberg B, Kim J, Gao WQ, Chen P. Qian D, et al. Dev Dyn. 2006 Jun;235(6):1689-700. doi: 10.1002/dvdy.20736. Dev Dyn. 2006. PMID: 16534784 Free PMC article.
• Characterization of the transcriptome of nascent hair cells and identification of direct targets of the Atoh1 transcription factor.
  Cai T, Jen HI, Kang H, Klisch TJ, Zoghbi HY, Groves AK. Cai T, et al. J Neurosci. 2015 Apr 8;35(14):5870-83. doi: 10.1523/JNEUROSCI.5083-14.2015. J Neurosci. 2015. PMID: 25855195 Free PMC article.
• A novel Atoh1 "self-terminating" mouse model reveals the necessity of proper Atoh1 level and duration for hair cell differentiation and viability.
  Pan N, Jahan I, Kersigo J, Duncan JS, Kopecky B, Fritzsch B. Pan N, et al. PLoS One. 2012;7(1):e30358. doi: 10.1371/journal.pone.0030358. Epub 2012 Jan 18. PLoS One. 2012. PMID: 22279587 Free PMC article.
• Development in the Mammalian Auditory System Depends on Transcription Factors.
  Elliott KL, Pavlínková G, Chizhikov VV, Yamoah EN, Fritzsch B. Elliott KL, et al. Int J Mol Sci. 2021 Apr 18;22(8):4189. doi: 10.3390/ijms22084189. Int J Mol Sci. 2021. PMID: 33919542 Free PMC article. Review.
• Atoh1: landscape for inner ear cell regeneration.
  Hongmiao R, Wei L, Bing H, Xiong DD, Jihao R. Hongmiao R, et al. Curr Gene Ther. 2014;14(2):101-11. doi: 10.2174/1566523214666140310143407. Curr Gene Ther. 2014. PMID: 24611726 Review.

Cited by

• Sodium-activated potassium channels shape peripheral auditory function and activity of the primary auditory neurons in mice.
  Reijntjes DOJ, Lee JH, Park S, Schubert NMA, van Tuinen M, Vijayakumar S, Jones TA, Jones SM, Gratton MA, Xia XM, Yamoah EN, Pyott SJ. Reijntjes DOJ, et al. Sci Rep. 2019 Feb 22;9(1):2573. doi: 10.1038/s41598-019-39119-z. Sci Rep. 2019. PMID: 30796290 Free PMC article.
• Recent advances in hair cell regeneration research.
  Collado MS, Burns JC, Hu Z, Corwin JT. Collado MS, et al. Curr Opin Otolaryngol Head Neck Surg. 2008 Oct;16(5):465-71. doi: 10.1097/MOO.0b013e32830f4ab5. Curr Opin Otolaryngol Head Neck Surg. 2008. PMID: 18797290 Free PMC article. Review.
• Integrated scRNAseq analyses of mouse cochlear supporting cells reveal the involvement of Ezh2 in hair cell regeneration.
  Zhao Y, Xu Z, Zhang R, Liu M, Lu C, Fan C, Wang J. Zhao Y, et al. Mol Biol Rep. 2024 Jan 28;51(1):217. doi: 10.1007/s11033-023-09173-y. Mol Biol Rep. 2024. PMID: 38281217
• Gene Expression by Mouse Inner Ear Hair Cells during Development.
  Scheffer DI, Shen J, Corey DP, Chen ZY. Scheffer DI, et al. J Neurosci. 2015 Apr 22;35(16):6366-80. doi: 10.1523/JNEUROSCI.5126-14.2015. J Neurosci. 2015. PMID: 25904789 Free PMC article.
• Genes expressed in Atoh1 neuronal lineages arising from the r1/isthmus rhombic lip.
  Machold R, Klein C, Fishell G. Machold R, et al. Gene Expr Patterns. 2011 Jun-Jul;11(5-6):349-59. doi: 10.1016/j.gep.2011.03.007. Epub 2011 Apr 2. Gene Expr Patterns. 2011. PMID: 21440680 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science
  • Wiley

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • BioCyc
  • Gene Ontology
  • Mouse Genome Informatics (MGI)