Nuclear envelope formation by chromatin-mediated reorganization of the endoplasmic reticulum - PubMed (original) (raw)

doi: 10.1038/ncb1636. Epub 2007 Sep 9.

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Nuclear envelope formation by chromatin-mediated reorganization of the endoplasmic reticulum

Daniel J Anderson et al. Nat Cell Biol. 2007 Oct.

Abstract

The formation of the nuclear envelope (NE) around chromatin is a major membrane-remodelling event that occurs during cell division of metazoa. It is unclear whether the nuclear membrane reforms by the fusion of NE fragments or if it re-emerges from an intact tubular network of the endoplasmic reticulum (ER). Here, we show that NE formation and expansion requires a tubular ER network and occurs efficiently in the presence of the membrane fusion inhibitor GTPgammaS. Chromatin recruitment of membranes, which is initiated by tubule-end binding, followed by the formation, expansion and sealing of flat membrane sheets, is mediated by DNA-binding proteins residing in the ER. Thus, chromatin plays an active role in reshaping of the ER during NE formation.

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