Differential expression of the keratan sulphate proteoglycan, keratocan, during chick corneal embryogenesis - PubMed (original) (raw)
Differential expression of the keratan sulphate proteoglycan, keratocan, during chick corneal embryogenesis
E Claire Gealy et al. Histochem Cell Biol. 2007 Dec.
Abstract
Keratan sulphate (KS) proteoglycans (PGs) are key molecules in the connective tissue matrix of the cornea of the eye, where they are believed to have functional roles in tissue organisation and transparency. Keratocan, is one of the three KS PGs expressed in cornea, and is the only one that is primarily cornea-specific. Work with the developing chick has shown that mRNA for keratocan is present in early corneal embryogenesis, but there is no evidence of protein synthesis and matrix deposition. Here, we investigate the tissue distribution of keratocan in the developing chick cornea as it becomes compacted and transparent in the later stages of development. Indirect immunofluorescence using a new monoclonal antibody (KER-1) which recognises a protein epitope on the keratocan core protein demonstrated that keratocan was present at all stages investigated (E10-E18), with distinct differences in localisation and organisation observed between early and later stages. Until E13, keratocan appeared both cell-associated and in the stromal extracellular matrix, and was particularly concentrated in superficial tissue regions. By E14 when the cornea begins to become transparent, keratocan was located in elongate arrays, presumably associated along collagen fibrils in the stroma. This fibrillar label was still concentrated in the anterior stroma, and persisted through E15-E18. Presumptive Bowman's layer was evident as an unlabelled subepithelial zone at all stages. Thus, in embryonic chick cornea, keratocan, in common with sulphated KS chains in the E12-E14 developmental period, exhibits a preferential distribution in the anterior stroma. It undergoes a striking reorganisation of structure and distribution consistent with a role in relation to stromal compaction and corneal transparency.
Similar articles
- Expression of the keratan sulfate proteoglycans lumican, keratocan and osteoglycin/mimecan during chick corneal development.
Dunlevy JR, Beales MP, Berryhill BL, Cornuet PK, Hassell JR. Dunlevy JR, et al. Exp Eye Res. 2000 Mar;70(3):349-62. doi: 10.1006/exer.1999.0789. Exp Eye Res. 2000. PMID: 10712821 - Keratocan, a cornea-specific keratan sulfate proteoglycan, is regulated by lumican.
Carlson EC, Liu CY, Chikama T, Hayashi Y, Kao CW, Birk DE, Funderburgh JL, Jester JV, Kao WW. Carlson EC, et al. J Biol Chem. 2005 Jul 8;280(27):25541-7. doi: 10.1074/jbc.M500249200. Epub 2005 Apr 22. J Biol Chem. 2005. PMID: 15849191 Free PMC article. - Keratocan-deficient mice display alterations in corneal structure.
Liu CY, Birk DE, Hassell JR, Kane B, Kao WW. Liu CY, et al. J Biol Chem. 2003 Jun 13;278(24):21672-7. doi: 10.1074/jbc.M301169200. Epub 2003 Mar 28. J Biol Chem. 2003. PMID: 12665512 - Roles of lumican and keratocan on corneal transparency.
Kao WW, Liu CY. Kao WW, et al. Glycoconj J. 2002 May-Jun;19(4-5):275-85. doi: 10.1023/A:1025396316169. Glycoconj J. 2002. PMID: 12975606 Review. - The molecular basis of corneal transparency.
Hassell JR, Birk DE. Hassell JR, et al. Exp Eye Res. 2010 Sep;91(3):326-35. doi: 10.1016/j.exer.2010.06.021. Epub 2010 Jul 3. Exp Eye Res. 2010. PMID: 20599432 Free PMC article. Review.
Cited by
- Keratan sulfate, an electrosensory neurosentient bioresponsive cell instructive glycosaminoglycan.
Melrose J. Melrose J. Glycobiology. 2024 Apr 1;34(3):cwae014. doi: 10.1093/glycob/cwae014. Glycobiology. 2024. PMID: 38376199 Free PMC article. Review. - Cell regulation of collagen fibril macrostructure during corneal morphogenesis.
Koudouna E, Mikula E, Brown DJ, Young RD, Quantock AJ, Jester JV. Koudouna E, et al. Acta Biomater. 2018 Oct 1;79:96-112. doi: 10.1016/j.actbio.2018.08.017. Epub 2018 Aug 29. Acta Biomater. 2018. PMID: 30170195 Free PMC article. - Glycosaminoglycans in the human cornea: age-related changes.
Pacella E, Pacella F, De Paolis G, Parisella FR, Turchetti P, Anello G, Cavallotti C. Pacella E, et al. Ophthalmol Eye Dis. 2015 Jan 27;7:1-5. doi: 10.4137/OED.S17204. eCollection 2015. Ophthalmol Eye Dis. 2015. PMID: 25674020 Free PMC article. - Murine corneal stroma cells inhibit LPS-induced dendritic cell maturation partially through TGF-β2 secretion in vitro.
Lu JM, Song XJ, Wang HF, Li XL, Zhang XR. Lu JM, et al. Mol Vis. 2012;18:2255-64. Epub 2012 Aug 10. Mol Vis. 2012. PMID: 22933838 Free PMC article.
References
- J Biochem. 1999 Nov;126(5):804-14 - PubMed
- Invest Ophthalmol Vis Sci. 2005 Nov;46(11):4046-9 - PubMed
- Biochem J. 1995 Feb 1;305 ( Pt 3):799-804 - PubMed
- J Cell Biol. 1998 Jun 1;141(5):1277-86 - PubMed
- Nat Genet. 2000 May;25(1):91-5 - PubMed
Publication types
MeSH terms
Substances
Grants and funding
- B18021/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom
- BBS/B/10994/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom
- 13279/ARC_/Arthritis Research UK/United Kingdom
- bbs/b/10994/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom
LinkOut - more resources
Full Text Sources