Nucleic acid renaturation and restriction endonuclease cleavage analyses show that the DNAs of a transforming and a nontransforming strain of Epstein-Barr virus share approximately 90% of their nucleotide sequences - PubMed (original) (raw)
Comparative Study
Nucleic acid renaturation and restriction endonuclease cleavage analyses show that the DNAs of a transforming and a nontransforming strain of Epstein-Barr virus share approximately 90% of their nucleotide sequences
B Sugden et al. J Virol. 1976 May.
Abstract
Viral DNA molecules were purified from a nontransforming and a transforming strain of Epstein-Barr virus. Each viral DNA was labeled in vitro and renatured in the presence of an excess of either one or the other unlabeled viral DNA. Both viral DNAs were also digested with the Eco R1 restriction endonuclease and subsequently labeled by using avian myeloblastosis virus DNA polymerase to repair either the EcoR1 nuclease-generated single-stranded ends of the DNAs or their single-stranded ends produced by a second digestion with exonuclease III after the first EcoR1 nuclease digestion. The results of these experiments support three general conclusions: (i) the DNAs of these two strains of Epstein-Barr virus share approximately 90% of their nucleotide sequences; (ii) both viral DNA populations are reasonably homogenous; and (iii) both DNAs contain repetitions or inverted repetitions of some of their nucleotide sequences.
Similar articles
- DNA of Epstein-Barr virus. IV. Linkage map of restriction enzyme fragments of the B95-8 and W91 strains of Epstein-Barr Virus.
Given D, Kieff E. Given D, et al. J Virol. 1978 Nov;28(2):524-42. doi: 10.1128/JVI.28.2.524-542.1978. J Virol. 1978. PMID: 214576 Free PMC article. - DNA of Epstein-Barr virus. V. Direct repeats of the ends of Epstein-Barr virus DNA.
Given D, Yee D, Griem K, Kieff E. Given D, et al. J Virol. 1979 Jun;30(3):852-62. doi: 10.1128/JVI.30.3.852-862.1979. J Virol. 1979. PMID: 225546 Free PMC article. - Heterogeneity of Epstein-Barr virus. III. Comparison of a transforming and a nontransforming virus by partial denaturation mapping of their DNAs.
Delius H, Bornkamm GW. Delius H, et al. J Virol. 1978 Jul;27(1):81-9. doi: 10.1128/JVI.27.1.81-89.1978. J Virol. 1978. PMID: 211256 Free PMC article. - [Structural organization of the Epstein-Barr virus genome].
D'iachenko AG. D'iachenko AG. Eksp Onkol. 1984;6(4):3-10. Eksp Onkol. 1984. PMID: 6209094 Review. Russian. - Mapping of putative transforming sequences of EBV DNA.
Kieff E, Raab-Traub N, Given D, King W, Powell AT, Pritchett R, Dambaugh T. Kieff E, et al. IARC Sci Publ (1971). 1978;(24 Pt 1):527-52. IARC Sci Publ (1971). 1978. PMID: 221358 Review.
Cited by
- Epstein-Barr virus with heterogeneous DNA disrupts latency.
Miller G, Rabson M, Heston L. Miller G, et al. J Virol. 1984 Apr;50(1):174-82. doi: 10.1128/JVI.50.1.174-182.1984. J Virol. 1984. PMID: 6321789 Free PMC article. - Identification of a rare Epstein-Barr virus variant that enhances early antigen expression in Raji cells.
Rabson M, Heston L, Miller G. Rabson M, et al. Proc Natl Acad Sci U S A. 1983 May;80(9):2762-6. doi: 10.1073/pnas.80.9.2762. Proc Natl Acad Sci U S A. 1983. PMID: 6302703 Free PMC article. - Non-immortalizing P3J-HR-1 Epstein-Barr virus: a deletion mutant of its transforming parent, Jijoye.
Rabson M, Gradoville L, Heston L, Miller G. Rabson M, et al. J Virol. 1982 Dec;44(3):834-44. doi: 10.1128/JVI.44.3.834-844.1982. J Virol. 1982. PMID: 6294333 Free PMC article. - Epstein-Barr virus induced proteins V: comparison of EBV-specific polypeptides from different virus strains.
Georg-Fries B, Mueller-Lantzsch N. Georg-Fries B, et al. Med Microbiol Immunol. 1982;171(1):11-21. doi: 10.1007/BF02122703. Med Microbiol Immunol. 1982. PMID: 6289060 - Association of the herpes simplex-1 viral gene for thymidine kinase with the human gene for adenylate kinase-1 in biochemically transformed cells.
Wilson DE, McKinlay MA, Staczek J, Whitkop C, Harrison B, Povey S. Wilson DE, et al. Biochem Genet. 1980 Oct;18(9-10):981-1001. doi: 10.1007/BF00500130. Biochem Genet. 1980. PMID: 6261733
References
- J Virol. 1967 Oct;1(5):1045-51 - PubMed
- Biochemistry. 1971 May 25;10(11):1993-2000 - PubMed
- Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 - PubMed
- J Mol Biol. 1975 Jan 25;91(3):315-28 - PubMed
- J Virol. 1975 Mar;15(3):556-9 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources