Structure of full-length HIV-1 CA: a model for the mature capsid lattice - PubMed (original) (raw)

. 2007 Oct 5;131(1):70-9.

doi: 10.1016/j.cell.2007.08.018.

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• PMID: 17923088
• DOI: 10.1016/j.cell.2007.08.018

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Structure of full-length HIV-1 CA: a model for the mature capsid lattice

Barbie K Ganser-Pornillos et al. Cell. 2007.

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Abstract

The capsids of mature retroviruses perform the essential function of organizing the viral genome for efficient replication. These capsids are modeled as fullerene structures composed of closed hexameric arrays of the viral CA protein, but a high-resolution structure of the lattice has remained elusive. A three-dimensional map of two-dimensional crystals of the R18L mutant of HIV-1 CA was derived by electron cryocrystallography. The docking of high-resolution domain structures into the map yielded the first unambiguous model for full-length HIV-1 CA. Three important protein-protein assembly interfaces are required for capsid formation. Each CA hexamer is composed of an inner ring of six N-terminal domains and an outer ring of C-terminal domains that form dimeric linkers connecting neighboring hexamers. Interactions between the two domains of CA further stabilize the hexamer and provide a structural explanation for the mechanism of action of known HIV-1 assembly inhibitors.

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Comment in

• How to assemble a capsid.
  Sundquist WI, Hill CP. Sundquist WI, et al. Cell. 2007 Oct 5;131(1):17-9. doi: 10.1016/j.cell.2007.09.028. Cell. 2007. PMID: 17923081
• Major Variations in HIV-1 Capsid Assembly Morphologies Involve Minor Variations in Molecular Structures of Structurally Ordered Protein Segments.
  Lu JX, Bayro MJ, Tycko R. Lu JX, et al. J Biol Chem. 2016 Jun 17;291(25):13098-112. doi: 10.1074/jbc.M116.720557. Epub 2016 Apr 19. J Biol Chem. 2016. PMID: 27129282 Free PMC article.

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