Microsequencing of proteins recorded in human two-dimensional gel protein databases - PubMed (original) (raw)
Microsequencing of proteins recorded in human two-dimensional gel protein databases
H H Rasmussen et al. Electrophoresis. 1991 Nov.
Abstract
Sixty-six human proteins recorded in the master transformed human epithelial amnion cells (AMA) (55) and keratinocyte (11) two-dimensional gel protein databases have been microsequenced since the last publication of the AMA database (Electrophoresis 1990, 12, 989-1071). Coomassie Brilliant Blue stained protein spots cut from several (up to 40) dry gels were concentrated by elution-concentration gel electrophoresis, electroblotted onto polyvinylidene difluoride membranes and in situ digested with trypsin. The eluting peptides were separated by reversed-phase high performance liquid chromatography (HPLC), collected individually and sequenced. Computer searches using the FASTA and TFASTA programs from the Genetics Computer Group indicated that 29 of the analyzed polypeptides correspond to hitherto unknown proteins.
Similar articles
- Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes.
Rasmussen HH, van Damme J, Puype M, Gesser B, Celis JE, Vandekerckhove J. Rasmussen HH, et al. Electrophoresis. 1992 Dec;13(12):960-9. doi: 10.1002/elps.11501301199. Electrophoresis. 1992. PMID: 1286667 - Development of a database of amino acid sequences for human colon carcinoma proteins separated by two-dimensional polyacrylamide gel electrophoresis.
Ward LD, Hong J, Whitehead RH, Simpson RJ. Ward LD, et al. Electrophoresis. 1990 Oct;11(10):883-91. doi: 10.1002/elps.1150111019. Electrophoresis. 1990. PMID: 2079031 - Comprehensive two-dimensional gel protein databases offer a global approach to the analysis of human cells: the transformed amnion cells (AMA) master database and its link to genome DNA sequence data.
Celis JE, Gesser B, Rasmussen HH, Madsen P, Leffers H, Dejgaard K, Honore B, Olsen E, Ratz G, Lauridsen JB, et al. Celis JE, et al. Electrophoresis. 1990 Dec;11(12):989-1071. doi: 10.1002/elps.1150111202. Electrophoresis. 1990. PMID: 2090460 - Sequence analysis of proteins separated by polyacrylamide gel electrophoresis: towards an integrated protein database.
Aebersold R, Leavitt J. Aebersold R, et al. Electrophoresis. 1990 Jul;11(7):517-27. doi: 10.1002/elps.1150110702. Electrophoresis. 1990. PMID: 2226408 Review. - Human cellular protein patterns and their link to genome DNA mapping and sequencing data: towards an integrated approach to the study of gene expression.
Celis JE, Rasmussen HH, Leffers H, Madsen P, Honoré B, Dejgaard K, Gromov P, Olsen E, Hoffmann HJ, Nielsen M, et al. Celis JE, et al. Genet Eng (N Y). 1993;15:21-40. doi: 10.1007/978-1-4899-1666-2_2. Genet Eng (N Y). 1993. PMID: 7763841 Review.
Cited by
- The maize two-dimensional gel protein database: towards an integrated genome analysis program.
Touzet P, Riccardi F, Morin C, Damerval C, Huet JC, Pernollet JC, Zivy M, de Vienne D. Touzet P, et al. Theor Appl Genet. 1996 Oct;93(5-6):997-1005. doi: 10.1007/BF00224104. Theor Appl Genet. 1996. PMID: 24162436 - Quantitative trait loci underlying gene product variation: a novel perspective for analyzing regulation of genome expression.
Damerval C, Maurice A, Josse JM, de Vienne D. Damerval C, et al. Genetics. 1994 May;137(1):289-301. doi: 10.1093/genetics/137.1.289. Genetics. 1994. PMID: 7914503 Free PMC article. - Cloning and expression in Escherichia coli of a dog thyroid cDNA encoding a novel inositol 1,4,5-trisphosphate 5-phosphatase.
Verjans B, De Smedt F, Lecocq R, Vanweyenberg V, Moreau C, Erneux C. Verjans B, et al. Biochem J. 1994 May 15;300 ( Pt 1)(Pt 1):85-90. doi: 10.1042/bj3000085. Biochem J. 1994. PMID: 8198557 Free PMC article. - Rapid purification and identification of calcyphosine, a Ca(2+)-binding protein phosphorylated by protein kinase A.
Lecocq R, Lamy F, Erneux C, Dumont JE. Lecocq R, et al. Biochem J. 1995 Feb 15;306 ( Pt 1)(Pt 1):147-51. doi: 10.1042/bj3060147. Biochem J. 1995. PMID: 7864802 Free PMC article. - Myosin heavy chain degradation during apoptosis in endothelial cells.
Suarez-Huerta N, Lecocq R, Mosselmans R, Galand P, Dumont JE, Robaye B. Suarez-Huerta N, et al. Cell Prolif. 2000 Apr;33(2):101-14. doi: 10.1046/j.1365-2184.2000.00169.x. Cell Prolif. 2000. PMID: 10845254 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources