Comparative expression of human endogenous retrovirus-W genes in multiple sclerosis - PubMed (original) (raw)
Comparative Study
. 2007 Oct;23(10):1251-6.
doi: 10.1089/aid.2006.0274.
Affiliations
- PMID: 17961112
- DOI: 10.1089/aid.2006.0274
Comparative Study
Comparative expression of human endogenous retrovirus-W genes in multiple sclerosis
Joseph M Antony et al. AIDS Res Hum Retroviruses. 2007 Oct.
Abstract
Human endogenous retroviruses (HERVs) have been associated with multiple sclerosis (MS) pathogenesis. Several related HERV-W sequences have been implicated in disease occurrence and progression; the MS retrovirus (MSRV) is one such element whose envelope protein has been recently demonstrated to be involved in innate immune pathogenesis. To distinguish MSRV from other HERV-W sequences we analyzed the relative abundance of individual HERV-W env sequences by employing a real-time PCR approach using specific oligonucleotide primers and tissue samples from MS and non-MS patients. Our analyses reveal that ERVWE1 env-encoding DNA and RNA exhibited increased detection (p < 0.05) and expression (p < 0.01) in the brains of MS patients. Similarly, ERVWE1 env transcripts were inducible in glial cells (p < 0.05), while comparable changes in MSRV abundance were not observed. These results indicate that individual HERVs might have distinct roles in MS pathogenesis.
Comment in
- Unreliable real-time PCR analysis of human endogenous retrovirus-W (HERV-W) RNA expression and DNA copy number in multiple sclerosis.
Garson JA, Huggett JF, Bustin SA, Pfaffl MW, Benes V, Vandesompele J, Shipley GL. Garson JA, et al. AIDS Res Hum Retroviruses. 2009 Mar;25(3):377-8; author reply 379-81. doi: 10.1089/aid.2008.0270. AIDS Res Hum Retroviruses. 2009. PMID: 19292592 No abstract available.
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