Polymorphism in Sirpa modulates engraftment of human hematopoietic stem cells - PubMed (original) (raw)

. 2007 Dec;8(12):1313-23.

doi: 10.1038/ni1527. Epub 2007 Nov 4.

Affiliations

• PMID: 17982459
• DOI: 10.1038/ni1527

Polymorphism in Sirpa modulates engraftment of human hematopoietic stem cells

Katsuto Takenaka et al. Nat Immunol. 2007 Dec.

Abstract

Graft failure in the transplantation of hematopoietic stem cells occurs despite donor-host genetic identity of human leukocyte antigens, suggesting that additional factors modulate engraftment. With the nobese diabetic (NOD)-severe combined immunodeficiency (SCID) xenotransplantation model, we found that the NOD background allowed better hematopoietic engraftment than did other strains with equivalent immunodeficiency-related mutations. We used positional genetics to characterize the molecular basis for this strain specificity and found that the NOD Sirpa allele conferred support for human hematopoiesis. NOD SIRP-alpha showed enhanced binding to the human CD47 ligand, and its expression on mouse macrophages was required for support of human hematopoiesis. Thus, we have identified Sirpa polymorphism as a potent genetic determinant of the engraftment of human hematopoietic stem cells.

PubMed Disclaimer

Comment in

• Macrophage tolerance: CD47-SIRP-alpha-mediated signals matter.
  Takizawa H, Manz MG. Takizawa H, et al. Nat Immunol. 2007 Dec;8(12):1287-9. doi: 10.1038/ni1207-1287. Nat Immunol. 2007. PMID: 18026079 No abstract available.

Similar articles

• The BALB/c-specific polymorphic SIRPA enhances its affinity for human CD47, inhibiting phagocytosis against human cells to promote xenogeneic engraftment.
  Iwamoto C, Takenaka K, Urata S, Yamauchi T, Shima T, Kuriyama T, Daitoku S, Saito Y, Miyamoto T, Iwasaki H, Kitabayashi I, Itoh K, Kishimoto J, Kohda D, Matozaki T, Akashi K. Iwamoto C, et al. Exp Hematol. 2014 Mar;42(3):163-171.e1. doi: 10.1016/j.exphem.2013.11.005. Epub 2013 Nov 20. Exp Hematol. 2014. PMID: 24269920
• A preclinical xenotransplantation animal model to assess human hematopoietic stem cell engraftment.
  Angelopoulou MK, Rinder H, Wang C, Burtness B, Cooper DL, Krause DS. Angelopoulou MK, et al. Transfusion. 2004 Apr;44(4):555-66. doi: 10.1111/j.1537-2995.2004.03285.x. Transfusion. 2004. PMID: 15043572
• Determination of engraftment potential of human cord blood stem-progenitor cells as a function of donor cell dosage and gestational age in the NOD/SCID mouse model.
  Ural SH, Sammel MD, Blakemore KJ. Ural SH, et al. Am J Obstet Gynecol. 2005 Sep;193(3 Pt 2):990-4. doi: 10.1016/j.ajog.2005.05.051. Am J Obstet Gynecol. 2005. PMID: 16157099
• Innate immune 'self' recognition: a role for CD47-SIRPalpha interactions in hematopoietic stem cell transplantation.
  van den Berg TK, van der Schoot CE. van den Berg TK, et al. Trends Immunol. 2008 May;29(5):203-6. doi: 10.1016/j.it.2008.02.004. Epub 2008 Apr 3. Trends Immunol. 2008. PMID: 18394962 Review.
• Rho GTPases and regulation of hematopoietic stem cell localization.
  Williams DA, Zheng Y, Cancelas JA. Williams DA, et al. Methods Enzymol. 2008;439:365-93. doi: 10.1016/S0076-6879(07)00427-2. Methods Enzymol. 2008. PMID: 18374178 Review.

Cited by

• Macrophage polarization and MRSA infection in burned mice.
  Nishiguchi T, Ito I, Lee JO, Suzuki S, Suzuki F, Kobayashi M. Nishiguchi T, et al. Immunol Cell Biol. 2017 Feb;95(2):198-206. doi: 10.1038/icb.2016.84. Epub 2016 Sep 6. Immunol Cell Biol. 2017. PMID: 27596946
• Heterogeneity in the CD200R paired receptor family.
  Akkaya M, Barclay AN. Akkaya M, et al. Immunogenetics. 2010 Jan;62(1):15-22. doi: 10.1007/s00251-009-0415-6. Epub 2009 Dec 5. Immunogenetics. 2010. PMID: 19967353
• Comparison of human fetal liver, umbilical cord blood, and adult blood hematopoietic stem cell engraftment in NOD-scid/gammac-/-, Balb/c-Rag1-/-gammac-/-, and C.B-17-scid/bg immunodeficient mice.
  Lepus CM, Gibson TF, Gerber SA, Kawikova I, Szczepanik M, Hossain J, Ablamunits V, Kirkiles-Smith N, Herold KC, Donis RO, Bothwell AL, Pober JS, Harding MJ. Lepus CM, et al. Hum Immunol. 2009 Oct;70(10):790-802. doi: 10.1016/j.humimm.2009.06.005. Epub 2009 Jun 12. Hum Immunol. 2009. PMID: 19524633 Free PMC article.
• Contractile forces sustain and polarize hematopoiesis from stem and progenitor cells.
  Shin JW, Buxboim A, Spinler KR, Swift J, Christian DA, Hunter CA, Léon C, Gachet C, Dingal PC, Ivanovska IL, Rehfeldt F, Chasis JA, Discher DE. Shin JW, et al. Cell Stem Cell. 2014 Jan 2;14(1):81-93. doi: 10.1016/j.stem.2013.10.009. Epub 2013 Nov 21. Cell Stem Cell. 2014. PMID: 24268694 Free PMC article.
• Establishing humanized mice using stem cells: maximizing the potential.
  Bernard D, Peakman M, Hayday AC. Bernard D, et al. Clin Exp Immunol. 2008 Jun;152(3):406-14. doi: 10.1111/j.1365-2249.2008.03659.x. Epub 2008 Apr 24. Clin Exp Immunol. 2008. PMID: 18435804 Free PMC article. Review.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Nature Publishing Group

• Other Literature Sources

  • H1 Connect - Access expert opinions and insights on biomedical research.
  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • Mouse Genome Informatics (MGI)

• Research Materials

  • NCI CPTC Antibody Characterization Program