Nuclear size control in fission yeast - PubMed (original) (raw)

Figure 1.

Nuclear and cell size correlate over a large range in cell size and shape. Quantitation of nuclear and cell size in cell size and cell shape mutants. (A) Overlay image of cell (differential interference contrast; DIC) and nuclear envelope (cut11-GFP). (B) Box-and-Whisker plots showing the N/C ratio. (C–E) Scatter plots of cell size and nuclear size. Wild type (WT): haploid (25°C, n = 382, r = 0.72), spores (25°C, n = 329. r = 0.63), nitrogen-starved cells (12 h EMM-N, 25°C, n = 275, r = 0.64), diploid (25°C, n = 308, r = 0.79). Cell size mutants: wee1-50, temperature-sensitive (ts) allele of the mitotic inhibitor wee1 resulting in a short G2 phase and mitosis at a smaller cell size (6 h 36°C, n = 179, r = 0.70), cdc10-V50, ts allele of a transcription factor controlling G1-S specific gene expression leading to a cell cycle arrest in G1 phase (3 h 36.5°C, n = 322, r = 0.82), cdc25-22, ts allele of the cdc25 phosphatase, an inducer of mitosis which arrests cells in G2 phase of the cell cycle (3 h 36.5°C, n = 188, r = 0.81), pnmt1-cdc13, a repressible allele of the b-type cyclin which leads to consecutive rounds of over-replication or arrest in G2 phase of the cell cycle (10 h release, n = 98, r = 0.62), orb2-34, a ts allele of PAK-related polarity kinase orb2 (5h 36.5°C, n = 122, r = 0.66), orb6-25, a ts allele of a kinase which coordinates cell morphogenesis with the cell cycle (5.5 h 36.5°C, n = 129, r = 0.69).