CD8 T cell control of HSV reactivation from latency is abrogated by viral inhibition of MHC class I - PubMed (original) (raw)
CD8 T cell control of HSV reactivation from latency is abrogated by viral inhibition of MHC class I
Mark T Orr et al. Cell Host Microbe. 2007.
Abstract
In humans, herpes simplex virus (HSV) establishes latency in sensory nerve ganglia from where it periodically reactivates, whereas in murine models, the virus efficiently establishes latency but rarely reactivates. HSV inhibits MHC class I antigen presentation to CD8 T cells efficiently in humans but poorly in mice, and whether this is a crucial determinant of HSV's ability to reactivate in humans remains uncertain. To test this, we generated a panel of recombinant HSVs that inhibit presentation by murine MHC class I mimicking the effect in humans. Antigen-specific CD8 T cells prevent the in vivo reactivation of wild-type HSV. Despite their presence in the ganglia of latently infected mice, CD8 T cells do not prevent the reactivation of recombinant HSVs that inhibit murine MHC class I in mice. These findings suggest that efficient inhibition of MHC class I by HSV is a key factor in its ability to reactivate in humans.
Figures
Figure 1. Viral inhibition of MHC class I does not prolong or prevent the clearance of primary infection
Female C57Bl/6 mice were infected with 2×105 pfu/eye of the indicated viruses. (A) 8 mice/virus were serially swabbed for viral shedding from both eyes on the indicated days. Alternatively, on the indicated days 4 mice/virus were euthanized and the (B) eyes and (C) trigeminal ganglia were harvested. Viral titers were determined by plaque formation on Vero cells. Statistically significant increases in viral titer compared to 27gfp on the same day are indicated (* p<0.05 and ** p<0.01). Error bars = one SEM. n.d. = not detected.
Figure 2. The amount of latent viral DNA is unaltered by viral inhibition of MHC class I
The relative quantity of viral DNA in the trigeminal ganglia of latently-infected mice 2 weeks post-infection was determined by realtime PCR for viral genomic DNA and normalized to the number of copies of the murine adipsin gene in each sample. 16-24 ganglia/virus were analyzed. Error bars = one SEM. Data are pooled from 2 experiments with similar results. There were no statistically significant differences among the groups.
Figure 3. Viral inhibition of MHC class I reduces the number and activation state of HSV-specific CD8 T cells in latently-infected ganglia
HSV gB-specific CD8 T cells in latently-infected trigeminal ganglia at (A) 14-16 or (B and C) 32-46 days post-infection were identified (A and B) by binding of H-2Kb DimerX loaded with HSV gB498-505 peptide or (C) by gB peptide-induced IFN-γ production. (D) The activation status of HSV gB-specific CD8 T cells was determined as the percentage of H-2Kb-gB DimerX-positive CD8 T cells that contained intracellular granzyme B directly ex vivo. The cells shown in the plots are gated on total cells (A, B and C) or (D) CD8 T cells in the trigeminal ganglia. The bar graphs indicate the mean percentage ± SEM (A, B and C) of CD8 T cells that are specific for HSV gB or (D) of HSV gB-specific CD8 T cells that express granzyme B ex vivo. Data are pooled from (A) three, (B) five, (C) four and (D) two experiments with similar results; in each experiment 8-10 ganglia/virus were analyzed. The difference between mice infected with 27m152 compared to 27gfp in each panel was statistically significant (P<0.0001). Error bars = one SEM.
Figure 4. 27m152 and 27US11 reactivate in the presence of HSV-specific CD8 T cells
Reactivation of rHSVs in vitro from explanted, latently-infected trigeminal ganglia in the presence of effector HSV-specific CD8 T cells plus IL-2 and (A) IgG2b isotype control or (B) α-CD8 monoclonal antibody (n = 8 trigeminal ganglia/group). (A) The increased frequencies of reactivation for 27US11 (P = 0.01) and 27m152 (P = 0.0017) compared to 27gfp were statistically significant. (B) The results for 27gfp and 27US11 data were identical and thus the symbols for 27US11 are not apparent in the figure.
Figure 5. Viral inhibition of MHC class I promotes reactivation in vivo
In vivo reactivation from the trigeminal ganglia and eyes of latently-infected mice following UV-exposure 5 weeks post-infection. (A) The numbers of recovered pfu’s from the trigeminal ganglia and eyes are shown. In some mice virus was recovered from both sites, in others only in the trigeminal ganglia. Virus was never recovered from the eyes but not the trigeminal ganglia. Data represent 18-19 mice/virus and were pooled from 2 experiments with similar results. (B) The overall frequency of reactivation in the trigeminal ganglia for each virus and treatment group is shown. Data are from 27-31 mice/virus pooled from 3 experiments, including the two experiments shown in A and a third experiment in which reactivation was determined on a combined homogenate of the ganglia and eyes. Statistically significant increases versus 27gfp are indicated (* p<0.05 and ** p<0.01).
Figure 6. Inhibition of MHC class I abrogates CD8 T cell prevention of HSV reactivation
In vivo reactivation of rHSV following UV-exposure in 8-10 latently-infected mice treated with α-CD8 or IgG2b isotype control Ab. (A) The numbers of recovered pfu’s from both the trigeminal ganglia and eyes are shown. In some mice virus was recovered from both sites, in others only from the trigeminal ganglia. Virus was never recovered from the eyes but not the trigeminal ganglia. (B) The overall frequency of reactivation in the trigeminal ganglia for each virus and treatment group is indicated. The results shown are from one of two experiments performed with similar results in both.
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