Induced pluripotent stem cell lines derived from human somatic cells - PubMed (original) (raw)

. 2007 Dec 21;318(5858):1917-20.

doi: 10.1126/science.1151526. Epub 2007 Nov 20.

Maxim A Vodyanik, Kim Smuga-Otto, Jessica Antosiewicz-Bourget, Jennifer L Frane, Shulan Tian, Jeff Nie, Gudrun A Jonsdottir, Victor Ruotti, Ron Stewart, Igor I Slukvin, James A Thomson

Affiliations

• PMID: 18029452
• DOI: 10.1126/science.1151526

Induced pluripotent stem cell lines derived from human somatic cells

Junying Yu et al. Science. 2007.

Abstract

Somatic cell nuclear transfer allows trans-acting factors present in the mammalian oocyte to reprogram somatic cell nuclei to an undifferentiated state. We show that four factors (OCT4, SOX2, NANOG, and LIN28) are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells. These induced pluripotent human stem cells have normal karyotypes, express telomerase activity, express cell surface markers and genes that characterize human ES cells, and maintain the developmental potential to differentiate into advanced derivatives of all three primary germ layers. Such induced pluripotent human cell lines should be useful in the production of new disease models and in drug development, as well as for applications in transplantation medicine, once technical limitations (for example, mutation through viral integration) are eliminated.

PubMed Disclaimer

Comment in

• Stem cell breakthrough: don't forget ethics.
  Lanza R. Lanza R. Science. 2007 Dec 21;318(5858):1865. doi: 10.1126/science.318.5858.1865a. Science. 2007. PMID: 18096789 No abstract available.
• Development. Is therapeutic cloning dead?
  Cibelli J. Cibelli J. Science. 2007 Dec 21;318(5858):1879-80. doi: 10.1126/science.1153229. Science. 2007. PMID: 18096796 No abstract available.
• Induced pluripotent cells mimicking human embryonic stem cells.
  Mohamadnejad M, Swenson ES. Mohamadnejad M, et al. Arch Iran Med. 2008 Jan;11(1):125-8. Arch Iran Med. 2008. PMID: 18154436 No abstract available.
• Achieving pluripotency.
  Baumann K. Baumann K. Nat Rev Mol Cell Biol. 2010 Oct;11(10):677. doi: 10.1038/nrm2980. Nat Rev Mol Cell Biol. 2010. PMID: 20861870 No abstract available.

Similar articles

• Induced pluripotent cells mimicking human embryonic stem cells.
  Mohamadnejad M, Swenson ES. Mohamadnejad M, et al. Arch Iran Med. 2008 Jan;11(1):125-8. Arch Iran Med. 2008. PMID: 18154436 No abstract available.
• Generation of human-induced pluripotent stem cells from gut mesentery-derived cells by ectopic expression of OCT4/SOX2/NANOG.
  Li Y, Zhao H, Lan F, Lee A, Chen L, Lin C, Yao Y, Li L. Li Y, et al. Cell Reprogram. 2010 Jun;12(3):237-47. doi: 10.1089/cell.2009.0103. Cell Reprogram. 2010. PMID: 20698766
• Reprogramming of human somatic cells to pluripotency with defined factors.
  Park IH, Zhao R, West JA, Yabuuchi A, Huo H, Ince TA, Lerou PH, Lensch MW, Daley GQ. Park IH, et al. Nature. 2008 Jan 10;451(7175):141-6. doi: 10.1038/nature06534. Epub 2007 Dec 23. Nature. 2008. PMID: 18157115
• The transcriptional network controlling pluripotency in ES cells.
  Orkin SH, Wang J, Kim J, Chu J, Rao S, Theunissen TW, Shen X, Levasseur DN. Orkin SH, et al. Cold Spring Harb Symp Quant Biol. 2008;73:195-202. doi: 10.1101/sqb.2008.72.001. Cold Spring Harb Symp Quant Biol. 2008. PMID: 19478325 Review.
• Transcriptional regulatory networks in embryonic stem cells.
  Chen X, Vega VB, Ng HH. Chen X, et al. Cold Spring Harb Symp Quant Biol. 2008;73:203-9. doi: 10.1101/sqb.2008.73.026. Epub 2008 Nov 6. Cold Spring Harb Symp Quant Biol. 2008. PMID: 19022762 Review.

Cited by

• Cell-based assay to detect small molecules restoring levels of let-7 miRNAs.
  Szewczyk S, Buckley B, Chernov M, Wang X, Pathak S, Yeger H, Attwood KM, Holtz R, Ambrosone CB, Higgins MJ. Szewczyk S, et al. Am J Cancer Res. 2024 Oct 15;14(10):4772-4787. doi: 10.62347/MBLD9480. eCollection 2024. Am J Cancer Res. 2024. PMID: 39553217 Free PMC article.
• Exploring dysfunctional barrier phenotypes associated with glaucoma using a human pluripotent stem cell-based model of the neurovascular unit.
  Lavekar SS, Hughes JM, Gomes C, Huang KC, Harkin J, Canfield SG, Meyer JS. Lavekar SS, et al. Fluids Barriers CNS. 2024 Nov 14;21(1):90. doi: 10.1186/s12987-024-00593-x. Fluids Barriers CNS. 2024. PMID: 39543684 Free PMC article.
• Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation.
  Matsuo-Takasaki M, Kambayashi S, Hemmi Y, Wakabayashi T, Shimizu T, An Y, Ito H, Takeuchi K, Ibuki M, Kawashima T, Masayasu R, Suzuki M, Kawai Y, Umekage M, Kato TM, Noguchi M, Nakade K, Nakamura Y, Nakaishi T, Nishishita N, Tsukahara M, Hayashi Y. Matsuo-Takasaki M, et al. Elife. 2024 Nov 12;12:RP89724. doi: 10.7554/eLife.89724. Elife. 2024. PMID: 39529479 Free PMC article.
• Application prospects of urine-derived stem cells in neurological and musculoskeletal diseases.
  Yang HS, Zheng YX, Bai X, He XY, Wang TH. Yang HS, et al. World J Orthop. 2024 Oct 18;15(10):918-931. doi: 10.5312/wjo.v15.i10.918. eCollection 2024 Oct 18. World J Orthop. 2024. PMID: 39473520 Free PMC article. Review.
• Reporter Alleles in hiPSCs: Visual Cues on Development and Disease.
  Cotta GC, Teixeira Dos Santos RC, Costa GMJ, Lacerda SMDSN. Cotta GC, et al. Int J Mol Sci. 2024 Oct 13;25(20):11009. doi: 10.3390/ijms252011009. Int J Mol Sci. 2024. PMID: 39456792 Free PMC article. Review.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Ovid Technologies, Inc.

• Other Literature Sources

  • H1 Connect
  • The Lens - Patent Citations Database

• Research Materials

  • Addgene Non-profit plasmid repository
  • Cellosaurus - a cell line knowledge resource
  • NCI CPTC Antibody Characterization Program