Structure of a nicked DNA-protein complex isolated from simian virus 40: covalent attachment of the protein to DNA and nick specificity - PubMed (original) (raw)

Structure of a nicked DNA-protein complex isolated from simian virus 40: covalent attachment of the protein to DNA and nick specificity

H Kasamatsu et al. Proc Natl Acad Sci U S A. 1976 Jun.

Abstract

A portion of the nicked circular DNA isolated from purified simian virus 40 contains a protein-DNA complex in which protein(s) is covalently attached to the end of a DNA single strand. (Nicked DNA is double-stranded DNA that contains at least one single-strand scission.) The protein was visualized by electron microscopy and labeled in vitro with 125I. The bond between the protein and the DNA is stable in alkali, 4 M guanidine-hydrochloride, 3.86 M hydroxylamine (pH 4,23), and in 98% formamide. Most of the molecules in the nicked circular DNA fraction contained one nick. The nick occurs on either of the two complementary strands; the specific nick sites on the two strands are staggered, but lie within a few hundred nucleotides of each other.

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References

1. 1. J Biol Chem. 1975 Nov 25;250(22):8796-803 - PubMed
2. 1. J Virol. 1975 Aug;16(2):366-87 - PubMed
3. 1. Proc Natl Acad Sci U S A. 1975 Jan;72(1):83-7 - PubMed
4. 1. Cold Spring Harb Symp Quant Biol. 1975;39 Pt 1:523-31 - PubMed
5. 1. Biochem Biophys Res Commun. 1976 Feb 9;68(3):927-36 - PubMed

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