Assembly, organization, and function of the COPII coat - PubMed (original) (raw)

Review

Assembly, organization, and function of the COPII coat

Helen Hughes et al. Histochem Cell Biol. 2008 Feb.

Abstract

A full mechanistic understanding of how secretory cargo proteins are exported from the endoplasmic reticulum for passage through the early secretory pathway is essential for us to comprehend how cells are organized, maintain compartment identity, as well as how they selectively secrete proteins and other macromolecules to the extracellular space. This process depends on the function of a multi-subunit complex, the COPII coat. Here we describe progress towards a full mechanistic understanding of COPII coat function, including the latest findings in this area. Much of our understanding of how COPII functions and is regulated comes from studies of yeast genetics, biochemical reconstitution and single cell microscopy. New developments arising from clinical cases and model organism biology and genetics enable us to gain far greater insight in to the role of membrane traffic in the context of a whole organism as well as during embryogenesis and development. A significant outcome of such a full understanding is to reveal how the machinery and processes of membrane trafficking through the early secretory pathway fail in disease states.

PubMed Disclaimer

Figures

Fig. 1

Fig. 1

Localization of COPII in mammalian cells. a Sec24C (COPII, green) and giantin (a marker of the cis-/medial-Golgi, red) in HeLa cells. Note the close proximity of the two structures in the juxtanuclear region results in apparent colocalization and yellow colouring in the merge. Bar 10 μm. b Sec24C (red) and calnexin (green) show the presence of ERES on ER membranes. Bar 10 μm. c Immunofluorescence labelling of methanol fixed HeLa cell shows the close apposition but distinct localization of Sec24C (COPII, green) and ERGIC-53 (red, a marker of the ER-Golgi intermediate compartment). Arrowheads highlight the clear separation of the two structures. Bar 5 μm. Individual green and red channels are shown alongside the merge for clarity

Fig. 2

Fig. 2

Schematic model for COPII–COPI coupling. The model shows the assembly and putative coupling of COPI and COPII function in mammalian cells. Starting from the top, the model shows the early stages of assembly of the COPII complex, dependent on Sar1 and Sec16 function. The blue box indicates events at the ERES and the protein components are labelled as detailed in the legend. This model is based on published work and includes known interactions. What is unclear is precisely how many of these interactions occur together i.e. just how large the complexes/subcomplexes can get. For example, we have no real idea whether there is a point at which Sec16 and COPI can be present on the same membrane/vesicle. Sec16 is drawn as part of the budded vesicle as the true fate of Sec16 during the COPII assembly/budding process remains unclear. At the stage of nascent VTC/tethering complex assembly (light blue) we have suggested that Sec13/31 dissociates. This is based on observations linking Sec23/24 to multiple important components that operate post budding such as TRAPPI and dynactin. The nascent VTC stage (light yellow) precedes complete loss of the COPII components, after which one is left with a COPI-coated VTC

Fig. 3

Fig. 3

Sequential assembly of the COPII coat structure. Following activation of Sar1-GDP to Sar1-GTP by the membrane-bound GEF Sec12, Sec23/24 is recruited to the ER membrane. Sec23/24 provides the major cargo binding function of the coat. Sec12 could be geometrically excluded from ERES because of membrane curvature. The final outer layer coat components Sec13/13 are recruited just prior to scission events, forming a vesicle. The minimal cage structure is shown; vesicle size would be dictated by cargo incorporation and potentially by other factors. These are schematic illustrations generated using Autodesk Maya 8.0 and are not intended as accurate rendering of the precise atomic coordinates from crystallographic data

Fig. 4

Fig. 4

Sec16 spatially restricts assembly of the COPII coat. a Outer coat components Sec13/31 form cuboctahedrons in their minimal state. The proline-rich region of Sec13 contacts Sec23/24 and Sar1 GTP stabilizing contacts between the inner and outer coat complexes. This also has the capacity to position Sec23/24 underneath the edges of the cage making the membrane surface (and associated proteins) more accessible. These are schematic illustrations generated using Autodesk Maya 8.0 and are not intended as accurate rendering of the precise atomic coordinates from crystallographic data

Similar articles

Cited by

References

    1. {'text': '', 'ref_index': 1, 'ids': [{'type': 'PubMed', 'value': '10903204', 'is_inner': True, 'url': 'https://pubmed.ncbi.nlm.nih.gov/10903204/'}\]}
    2. Allan BB, Moyer BD, Balch WE (2000) Rab1 recruitment of p115 into a cis-SNARE complex: programming budding COPII vesicles for fusion. Science 289:444–448 - PubMed
    1. {'text': '', 'ref_index': 1, 'ids': [{'type': 'PMC', 'value': 'PMC2168100', 'is_inner': False, 'url': 'https://pmc.ncbi.nlm.nih.gov/articles/PMC2168100/'}, {'type': 'PubMed', 'value': '10601335', 'is_inner': True, 'url': 'https://pubmed.ncbi.nlm.nih.gov/10601335/'}\]}
    2. Alvarez C, Fujita H, Hubbard A, Sztul E (1999) ER to Golgi transport: requirement for p115 at a pre-Golgi VTC stage. J Cell Biol 147:1205–1222 - PMC - PubMed
    1. {'text': '', 'ref_index': 1, 'ids': [{'type': 'PMC', 'value': 'PMC165101', 'is_inner': False, 'url': 'https://pmc.ncbi.nlm.nih.gov/articles/PMC165101/'}, {'type': 'PubMed', 'value': '12802079', 'is_inner': True, 'url': 'https://pubmed.ncbi.nlm.nih.gov/12802079/'}\]}
    2. Alvarez C, Garcia-Mata R, Brandon E, Sztul E (2003) COPI Recruitment is modulated by a Rab1b-dependent mechanism. Mol Biol Cell 14:2116–2127 - PMC - PubMed
    1. {'text': '', 'ref_index': 1, 'ids': [{'type': 'PubMed', 'value': '11389436', 'is_inner': True, 'url': 'https://pubmed.ncbi.nlm.nih.gov/11389436/'}\]}
    2. Antonny B, Madden D, Hamamoto S, Orci L, Schekman R (2001) Dynamics of the COPII coat with GTP and stable analogues. Nat Cell Biol 3:531–537 - PubMed
    1. {'text': '', 'ref_index': 1, 'ids': [{'type': 'PMC', 'value': 'PMC1319167', 'is_inner': False, 'url': 'https://pmc.ncbi.nlm.nih.gov/articles/PMC1319167/'}, {'type': 'PubMed', 'value': '12671686', 'is_inner': True, 'url': 'https://pubmed.ncbi.nlm.nih.gov/12671686/'}\]}
    2. Antonny B, Gounon P, Schekman R, Orci L (2003) Self-assembly of minimal COPII cages. EMBO Rep 4:419–424 - PMC - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources