Chromatin remodelling at promoters suppresses antisense transcription - PubMed (original) (raw)
. 2007 Dec 13;450(7172):1031-5.
doi: 10.1038/nature06391.
Affiliations
- PMID: 18075583
- DOI: 10.1038/nature06391
Chromatin remodelling at promoters suppresses antisense transcription
Iestyn Whitehouse et al. Nature. 2007.
Abstract
Chromatin allows the eukaryotic cell to package its DNA efficiently. To understand how chromatin structure is controlled across the Saccharomyces cerevisiae genome, we have investigated the role of the ATP-dependent chromatin remodelling complex Isw2 in positioning nucleosomes. We find that Isw2 functions adjacent to promoter regions where it repositions nucleosomes at the interface between genic and intergenic sequences. Nucleosome repositioning by Isw2 is directional and results in increased nucleosome occupancy of the intergenic region. Loss of Isw2 activity leads to inappropriate transcription, resulting in the generation of both coding and noncoding transcripts. Here we show that Isw2 repositions nucleosomes to enforce directionality on transcription by preventing transcription initiation from cryptic sites. Our analyses reveal how chromatin is organized on a global scale and advance our understanding of how transcription is regulated.
Comment in
- Molecular biology: genome under surveillance.
Arndt KM. Arndt KM. Nature. 2007 Dec 13;450(7172):959-60. doi: 10.1038/450959a. Nature. 2007. PMID: 18075570 No abstract available.
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