Tead4 is required for specification of trophectoderm in pre-implantation mouse embryos - PubMed (original) (raw)
. 2008 Mar-Apr;125(3-4):270-83.
doi: 10.1016/j.mod.2007.11.002. Epub 2007 Nov 17.
Affiliations
- PMID: 18083014
- DOI: 10.1016/j.mod.2007.11.002
Free article
Tead4 is required for specification of trophectoderm in pre-implantation mouse embryos
Noriyuki Nishioka et al. Mech Dev. 2008 Mar-Apr.
Free article
Abstract
During pre-implantation mouse development, embryos form blastocysts with establishment of the first two cell lineages: the trophectoderm (TE) which gives rise to the placenta, and the inner cell mass (ICM) which will form the embryo proper. Differentiation of TE is regulated by the transcription factor Caudal-related homeobox 2 (Cdx2), but the mechanisms which act upstream of Cdx2 expression remain unknown. Here we show that the TEA domain family transcription factor, Tead4, is required for TE development. Tead1, Tead2 and Tead4 were expressed in pre-implantation embryos, and at least Tead1 and Tead4 were expressed widely in both TE and ICM lineages. Tead4-/- embryos died at pre-implantation stages without forming the blastocoel. The mutant embryos continued cell proliferation, and adherens junction and cell polarity were not significantly affected. In Tead4-/- embryos, Cdx2 was weakly expressed at the morula stage but was not expressed in later stages. None of the TE specific genes, including Eomes and a Cdx2 independent gene, Fgfr2, was detected in Tead4-/- embryos. Instead, the ICM specific transcription factors, Oct3/4 and Nanog, were expressed in all the blastomeres. Tead4-/- embryos also failed to differentiate trophoblast giant cells when they were cultured in vitro. ES cells with normal in vitro differentiation abilities were established from Tead4-/- embryos. These results suggest that Tead4 has a distinct role from Tead1 and Tead2 in trophectoderm specification of pre-implantation embryos, and that Tead4 is an early transcription factor required for specification and development of the trophectoderm lineage, which includes expression of Cdx2.
Similar articles
- TEAD4 regulates trophectoderm differentiation upstream of CDX2 in a GATA3-independent manner in the human preimplantation embryo.
Stamatiadis P, Cosemans G, Boel A, Menten B, De Sutter P, Stoop D, Chuva de Sousa Lopes SM, Lluis F, Coucke P, Heindryckx B. Stamatiadis P, et al. Hum Reprod. 2022 Jul 30;37(8):1760-1773. doi: 10.1093/humrep/deac138. Hum Reprod. 2022. PMID: 35700449 - Cdx2 is required for correct cell fate specification and differentiation of trophectoderm in the mouse blastocyst.
Strumpf D, Mao CA, Yamanaka Y, Ralston A, Chawengsaksophak K, Beck F, Rossant J. Strumpf D, et al. Development. 2005 May;132(9):2093-102. doi: 10.1242/dev.01801. Epub 2005 Mar 23. Development. 2005. PMID: 15788452 - Lineage segregation in human pre-implantation embryos is specified by YAP1 and TEAD1.
Regin M, Essahib W, Demtschenko A, Dewandre D, David L, Gerri C, Niakan KK, Verheyen G, Tournaye H, Sterckx J, Sermon K, Van De Velde H. Regin M, et al. Hum Reprod. 2023 Aug 1;38(8):1484-1498. doi: 10.1093/humrep/dead107. Hum Reprod. 2023. PMID: 37295962 - Mechanisms of trophectoderm fate specification in preimplantation mouse development.
Sasaki H. Sasaki H. Dev Growth Differ. 2010 Apr;52(3):263-73. doi: 10.1111/j.1440-169X.2009.01158.x. Epub 2010 Jan 20. Dev Growth Differ. 2010. PMID: 20100249 Review. - Establishment of trophectoderm and inner cell mass lineages in the mouse embryo.
Marikawa Y, Alarcón VB. Marikawa Y, et al. Mol Reprod Dev. 2009 Nov;76(11):1019-32. doi: 10.1002/mrd.21057. Mol Reprod Dev. 2009. PMID: 19479991 Free PMC article. Review.
Cited by
- TEAD transcription factor family emerges as a promising therapeutic target for oral squamous cell carcinoma.
Wang S, Shao D, Gao X, Zhao P, Kong F, Deng J, Yang L, Shang W, Sun Y, Fu Z. Wang S, et al. Front Immunol. 2024 Oct 4;15:1480701. doi: 10.3389/fimmu.2024.1480701. eCollection 2024. Front Immunol. 2024. PMID: 39430767 Free PMC article. Review. - Roles of cell differentiation factors in preimplantation development of domestic animals.
Sawai K. Sawai K. J Reprod Dev. 2021 Jun 21;67(3):161-165. doi: 10.1262/jrd.2021-031. Epub 2021 Apr 27. J Reprod Dev. 2021. PMID: 33907058 Free PMC article. Review. - Transcription factor AP-2γ induces early Cdx2 expression and represses HIPPO signaling to specify the trophectoderm lineage.
Cao Z, Carey TS, Ganguly A, Wilson CA, Paul S, Knott JG. Cao Z, et al. Development. 2015 May 1;142(9):1606-15. doi: 10.1242/dev.120238. Epub 2015 Apr 9. Development. 2015. PMID: 25858457 Free PMC article. - Cytoskeletal control of early mammalian development.
Lim HYG, Plachta N. Lim HYG, et al. Nat Rev Mol Cell Biol. 2021 Aug;22(8):548-562. doi: 10.1038/s41580-021-00363-9. Epub 2021 Apr 29. Nat Rev Mol Cell Biol. 2021. PMID: 33927361 Review. - Building Principles for Constructing a Mammalian Blastocyst Embryo.
Pfeffer PL. Pfeffer PL. Biology (Basel). 2018 Jul 23;7(3):41. doi: 10.3390/biology7030041. Biology (Basel). 2018. PMID: 30041494 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Research Materials
Miscellaneous