The Mucin degrader Akkermansia muciniphila is an abundant resident of the human intestinal tract - PubMed (original) (raw)

Comparative Study

The Mucin degrader Akkermansia muciniphila is an abundant resident of the human intestinal tract

Muriel Derrien et al. Appl Environ Microbiol. 2008 Mar.

Abstract

A 16S rRNA-targeted probe, MUC-1437, was designed and validated in order to determine the presence and numbers of cells of Akkermansia muciniphila, a mucin degrader, in the human intestinal tract. As determined by fluorescent in situ hybridization, A. muciniphila accounted more than 1% of the total fecal cells and was shown to be a common bacterial component of the human intestinal tract.

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Figures

FIG. 1.

FIG. 1.

Phylogenetic tree showing the position of A. muciniphila and the clone sequences in the Verrucomicrobia phylum. The numbers after the commas are accession numbers. Bar = 10% sequence divergence.

FIG. 2.

FIG. 2.

Quantification of A. muciniphila (log cells/g [wet weight] of feces) in feces from Finnish (F) babies and adults and Dutch (D) adults, with the results represented by box and whisker plots. Each box extends from the 25th percentile to the 75th percentile, and a line indicates the median. The whiskers show the highest and lowest values after exclusion of the outliers, which are represented by dots.

References

    1. Allen, A. 1983. Mucus—a protective secretion of complexity. Trends Biochem. Sci. 8:169-173.
    1. Alm, E., D. Oerther, N. Larsen, D. Stahl, and L. Raskin. 1996. The oligonucleotide probe database. Appl. Environ. Microbiol. 62:3557-3559. - PMC - PubMed
    1. Amann, R., L. Krumholz, and D. Stahl. 1990. Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology. J. Bacteriol. 172:762-770. - PMC - PubMed
    1. Cole, J. R., B. Chai, R. J. Farris, Q. Wang, S. A. Kulam, D. M. McGarrell, G. M. Garrity, and J. M. Tiedje. 2005. The Ribosomal Database Project (RDP-II): sequences and tools for high-throughput rRNA analysis. Nucleic Acids Res. 33:D294-D296. - PMC - PubMed
    1. Collado, M. C., M. Derrien, E. Isolauri, W. de Vos, and S. Salminen. 2007. Intestinal integrity and Akkermansia muciniphila, a mucin-degrading member of the intestinal microbiota present in infants, adults, and the elderly. Appl. Environ. Microbiol. 73:7767-7770. - PMC - PubMed

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