The actin-related protein hArp8 accumulates on the mitotic chromosomes and functions in chromosome alignment - PubMed (original) (raw)
. 2008 Feb 15;314(4):859-68.
doi: 10.1016/j.yexcr.2007.11.020. Epub 2007 Dec 4.
Affiliations
- PMID: 18163988
- DOI: 10.1016/j.yexcr.2007.11.020
The actin-related protein hArp8 accumulates on the mitotic chromosomes and functions in chromosome alignment
Naoki Aoyama et al. Exp Cell Res. 2008.
Abstract
The actin family consists of conventional actin and various actin-related proteins (Arps). Some of these Arps are localized in the nucleus, and a fraction of each of these nuclear Arps is functionally involved in chromatin remodeling and histone acetyltransferase complexes. On the other hand, in mitotic cells, the localization and function of the nuclear Arps are largely unknown. Human Arp8 (hArp8), an ortholog of yeast nuclear Arp8, was recently found to be associated with the hINO80-chromatin remodeling complex along with hArp5. Here we report that hArp8, but not hArp5, accumulates on mitotic chromosomes. This is the first example where a member of the actin family is found to be associated with mitotic chromosomes. Expression of truncated hArp8 proteins and depletion of endogenous hArp8 by RNA interference caused misalignment of mitotic chromosomes, suggesting that chromosome-associated hArp8 has a role in chromosome behavior. In contrast, depletion of hIno80 and hArp5 did not cause misalignment of chromosomes, suggesting that the role of hArp8 at mitotic chromosomes is independent of the activity of hINO80 complexes. These findings provide the first insight into a novel function of actin family members in mitosis.
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