Structural insights into the exon junction complex - PubMed (original) (raw)
Review
Structural insights into the exon junction complex
Hervé Le Hir et al. Curr Opin Struct Biol. 2008 Feb.
Abstract
In higher eukaryotes, the exon junction complex is loaded onto spliced mRNAs at a precise position upstream of exon junctions, where it remains during nuclear export and cytoplasmic localisation until it is removed during the first translation round. The exon junction core complex consists of four proteins that form a dynamic binding platform for a variety of peripheral factors involved in mRNA metabolism. In the complex, mRNA binding is mediated by the DEAD-box protein eIF4AIII, and inhibition of its ATPase activity forms the mechanistic basis for the long-term stability of the complex. Recent crystal structures of the exon junction complex and eIF4AIII have provided the structural framework for investigating the function of the eIF4AIII ATPase and for localisation of surface patches involved in binding peripheral factors. Additionally, by comparison with the structure of a second DEAD-box protein also bound to RNA and ATP, general principles for the ATPase and unwinding/mRNP remodelling activities for this important group of enzymes can be proposed on the basis of atomic structures.
Similar articles
- Structure of the exon junction core complex with a trapped DEAD-box ATPase bound to RNA.
Andersen CB, Ballut L, Johansen JS, Chamieh H, Nielsen KH, Oliveira CL, Pedersen JS, Séraphin B, Le Hir H, Andersen GR. Andersen CB, et al. Science. 2006 Sep 29;313(5795):1968-72. doi: 10.1126/science.1131981. Epub 2006 Aug 24. Science. 2006. PMID: 16931718 - The crystal structure of the exon junction complex reveals how it maintains a stable grip on mRNA.
Bono F, Ebert J, Lorentzen E, Conti E. Bono F, et al. Cell. 2006 Aug 25;126(4):713-25. doi: 10.1016/j.cell.2006.08.006. Cell. 2006. PMID: 16923391 - The exon junction core complex is locked onto RNA by inhibition of eIF4AIII ATPase activity.
Ballut L, Marchadier B, Baguet A, Tomasetto C, Séraphin B, Le Hir H. Ballut L, et al. Nat Struct Mol Biol. 2005 Oct;12(10):861-9. doi: 10.1038/nsmb990. Epub 2005 Sep 18. Nat Struct Mol Biol. 2005. PMID: 16170325 - The ever-increasing complexities of the exon junction complex.
Tange TØ, Nott A, Moore MJ. Tange TØ, et al. Curr Opin Cell Biol. 2004 Jun;16(3):279-84. doi: 10.1016/j.ceb.2004.03.012. Curr Opin Cell Biol. 2004. PMID: 15145352 Review. - Messenger-RNA-binding proteins and the messages they carry.
Dreyfuss G, Kim VN, Kataoka N. Dreyfuss G, et al. Nat Rev Mol Cell Biol. 2002 Mar;3(3):195-205. doi: 10.1038/nrm760. Nat Rev Mol Cell Biol. 2002. PMID: 11994740 Review.
Cited by
- The Exon Junction Complex Factor RBM8A in Glial Fibrillary Acid Protein-Expressing Astrocytes Modulates Locomotion Behaviors.
Asthana S, Mott J, Tong M, Pei Z, Mao Y. Asthana S, et al. Cells. 2024 Mar 13;13(6):498. doi: 10.3390/cells13060498. Cells. 2024. PMID: 38534343 Free PMC article. - Rbm8a deficiency causes hematopoietic defects by modulating Wnt/PCP signaling.
Kocere A, Chiavacci E, Soneson C, Wells HH, Méndez-Acevedo KM, MacGowan JS, Jacobson ST, Hiltabidle MS, Raghunath A, Shavit JA, Panáková D, Williams MLK, Robinson MD, Mosimann C, Burger A. Kocere A, et al. bioRxiv [Preprint]. 2024 Feb 1:2023.04.12.536513. doi: 10.1101/2023.04.12.536513. bioRxiv. 2024. PMID: 37090609 Free PMC article. Preprint. - Sequence-specific remodeling of a topologically complex RNP substrate by Spb4.
Cruz VE, Sekulski K, Peddada N, Sailer C, Balasubramanian S, Weirich CS, Stengel F, Erzberger JP. Cruz VE, et al. Nat Struct Mol Biol. 2022 Dec;29(12):1228-1238. doi: 10.1038/s41594-022-00874-9. Epub 2022 Dec 8. Nat Struct Mol Biol. 2022. PMID: 36482249 Free PMC article. - Enteroviral 2C protein is an RNA-stimulated ATPase and uses a two-step mechanism for binding to RNA and ATP.
Yeager C, Carter G, Gohara DW, Yennawar NH, Enemark EJ, Arnold JJ, Cameron CE. Yeager C, et al. Nucleic Acids Res. 2022 Nov 11;50(20):11775-11798. doi: 10.1093/nar/gkac1054. Nucleic Acids Res. 2022. PMID: 36399514 Free PMC article. - Diverse Roles of the Exon Junction Complex Factors in the Cell Cycle, Cancer, and Neurodevelopmental Disorders-Potential for Therapeutic Targeting.
Martin H, Rupkey J, Asthana S, Yoon J, Patel S, Mott J, Pei Z, Mao Y. Martin H, et al. Int J Mol Sci. 2022 Sep 8;23(18):10375. doi: 10.3390/ijms231810375. Int J Mol Sci. 2022. PMID: 36142288 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources