High-density mapping of single-molecule trajectories with photoactivated localization microscopy - PubMed (original) (raw)
doi: 10.1038/nmeth.1176. Epub 2008 Jan 13.
Affiliations
- PMID: 18193054
- DOI: 10.1038/nmeth.1176
Free article
High-density mapping of single-molecule trajectories with photoactivated localization microscopy
Suliana Manley et al. Nat Methods. 2008 Feb.
Free article
Abstract
We combined photoactivated localization microscopy (PALM) with live-cell single-particle tracking to create a new method termed sptPALM. We created spatially resolved maps of single-molecule motions by imaging the membrane proteins Gag and VSVG, and obtained several orders of magnitude more trajectories per cell than traditional single-particle tracking enables. By probing distinct subsets of molecules, sptPALM can provide insight into the origins of spatial and temporal heterogeneities in membranes.
Comment in
- How the sum of its parts gets greater than the whole.
Brameshuber M, Schütz GJ. Brameshuber M, et al. Nat Methods. 2008 Feb;5(2):133-4. doi: 10.1038/nmeth0208-133. Nat Methods. 2008. PMID: 18235433 No abstract available.
Similar articles
- Single-particle tracking photoactivated localization microscopy of membrane proteins in living plant tissues.
Bayle V, Fiche JB, Burny C, Platre MP, Nollmann M, Martinière A, Jaillais Y. Bayle V, et al. Nat Protoc. 2021 Mar;16(3):1600-1628. doi: 10.1038/s41596-020-00471-4. Epub 2021 Feb 24. Nat Protoc. 2021. PMID: 33627844 - Single-particle tracking photoactivated localization microscopy for mapping single-molecule dynamics.
Manley S, Gillette JM, Lippincott-Schwartz J. Manley S, et al. Methods Enzymol. 2010;475:109-20. doi: 10.1016/S0076-6879(10)75005-9. Methods Enzymol. 2010. PMID: 20627155 Free PMC article. - Bright monomeric photoactivatable red fluorescent protein for two-color super-resolution sptPALM of live cells.
Subach FV, Patterson GH, Renz M, Lippincott-Schwartz J, Verkhusha VV. Subach FV, et al. J Am Chem Soc. 2010 May 12;132(18):6481-91. doi: 10.1021/ja100906g. J Am Chem Soc. 2010. PMID: 20394363 Free PMC article. - Optical techniques for imaging membrane domains in live cells (live-cell palm of protein clustering).
Owen DM, Williamson D, Magenau A, Gaus K. Owen DM, et al. Methods Enzymol. 2012;504:221-35. doi: 10.1016/B978-0-12-391857-4.00011-2. Methods Enzymol. 2012. PMID: 22264537 Review. - Tracking of single receptor molecule mobility in neuronal membranes: a quick theoretical and practical guide.
Kwakowsky A, Potapov D, Abrahám IM. Kwakowsky A, et al. J Neuroendocrinol. 2013 Nov;25(11):1231-7. doi: 10.1111/jne.12083. J Neuroendocrinol. 2013. PMID: 23927034 Review.
Cited by
- BDNF-dependent nano-organization of Neogenin and the WAVE regulatory complex promotes actin remodeling in dendritic spines.
Shohayeb B, Sempert K, Wallis TP, Meunier FA, Durisic N, O'Brien EA, Flores C, Cooper HM. Shohayeb B, et al. iScience. 2024 Jul 30;27(9):110621. doi: 10.1016/j.isci.2024.110621. eCollection 2024 Sep 20. iScience. 2024. PMID: 39228790 Free PMC article. - High-throughput super-resolution single-particle trajectory analysis reconstructs organelle dynamics and membrane reorganization.
Parutto P, Heck J, Lu M, Kaminski C, Avezov E, Heine M, Holcman D. Parutto P, et al. Cell Rep Methods. 2022 Aug 22;2(8):100277. doi: 10.1016/j.crmeth.2022.100277. eCollection 2022 Aug 22. Cell Rep Methods. 2022. PMID: 36046627 Free PMC article. - Improving spatial precision and field-of-view in wavelength-tagged single-particle tracking using spectroscopic single-molecule localization microscopy.
Brenner B, Song KH, Sun C, Zhang HF. Brenner B, et al. Appl Opt. 2021 May 1;60(13):3647-3658. doi: 10.1364/AO.415275. Appl Opt. 2021. PMID: 33983297 Free PMC article. - Spatiotemporal Fluctuation Analysis: A Powerful Tool for the Future Nanoscopy of Molecular Processes.
Di Rienzo C, Gratton E, Beltram F, Cardarelli F. Di Rienzo C, et al. Biophys J. 2016 Aug 23;111(4):679-685. doi: 10.1016/j.bpj.2016.07.015. Biophys J. 2016. PMID: 27558712 Free PMC article. - Monitoring cell membrane recycling dynamics of proteins using whole-cell fluorescence recovery after photobleaching of pH-sensitive genetic tags.
Michaluk P, Rusakov DA. Michaluk P, et al. Nat Protoc. 2022 Dec;17(12):3056-3079. doi: 10.1038/s41596-022-00732-4. Epub 2022 Sep 5. Nat Protoc. 2022. PMID: 36064755 Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources