High-density mapping of single-molecule trajectories with photoactivated localization microscopy - PubMed (original) (raw)
doi: 10.1038/nmeth.1176. Epub 2008 Jan 13.
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- PMID: 18193054
- DOI: 10.1038/nmeth.1176
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High-density mapping of single-molecule trajectories with photoactivated localization microscopy
Suliana Manley et al. Nat Methods. 2008 Feb.
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Abstract
We combined photoactivated localization microscopy (PALM) with live-cell single-particle tracking to create a new method termed sptPALM. We created spatially resolved maps of single-molecule motions by imaging the membrane proteins Gag and VSVG, and obtained several orders of magnitude more trajectories per cell than traditional single-particle tracking enables. By probing distinct subsets of molecules, sptPALM can provide insight into the origins of spatial and temporal heterogeneities in membranes.
Comment in
- How the sum of its parts gets greater than the whole.
Brameshuber M, Schütz GJ. Brameshuber M, et al. Nat Methods. 2008 Feb;5(2):133-4. doi: 10.1038/nmeth0208-133. Nat Methods. 2008. PMID: 18235433 No abstract available.
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