High-density mapping of single-molecule trajectories with photoactivated localization microscopy - PubMed (original) (raw)

doi: 10.1038/nmeth.1176. Epub 2008 Jan 13.

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High-density mapping of single-molecule trajectories with photoactivated localization microscopy

Suliana Manley et al. Nat Methods. 2008 Feb.

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Abstract

We combined photoactivated localization microscopy (PALM) with live-cell single-particle tracking to create a new method termed sptPALM. We created spatially resolved maps of single-molecule motions by imaging the membrane proteins Gag and VSVG, and obtained several orders of magnitude more trajectories per cell than traditional single-particle tracking enables. By probing distinct subsets of molecules, sptPALM can provide insight into the origins of spatial and temporal heterogeneities in membranes.

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