UAP56 RNA helicase is required for axis specification and cytoplasmic mRNA localization in Drosophila - PubMed (original) (raw)
. 2008 Mar 1;315(1):89-98.
doi: 10.1016/j.ydbio.2007.12.004. Epub 2007 Dec 14.
Affiliations
- PMID: 18237727
- DOI: 10.1016/j.ydbio.2007.12.004
Free article
UAP56 RNA helicase is required for axis specification and cytoplasmic mRNA localization in Drosophila
Carine Meignin et al. Dev Biol. 2008.
Free article
Abstract
mRNA export from the nucleus requires the RNA helicase UAP56 and involves remodeling of ribonucleo-protein complexes in the nucleus. Here, we show that UAP56 is required for bulk mRNA export from the nurse cell nuclei that supply most of the material to the growing Drosophila oocyte and for the organization of chromatin in the oocyte nucleus. Loss of UAP56 function leads to patterning defects that identify uap56 as a spindle-class gene similar to the RNA helicase Vasa. UAP56 is required for the localization of gurken, bicoid and oskar mRNA as well as post-translational modification of Osk protein. By injecting grk RNA into the oocyte cytoplasm, we show that UAP56 plays a role in cytoplasmic mRNA localization. We propose that UAP56 has two independent functions in the remodeling of ribonucleo-protein complexes. The first is in the nucleus for mRNA export of most transcripts from the nucleus. The second is in the cytoplasm for remodeling the transacting factors that decorate mRNA and dictate its cytoplasmic destination.
Similar articles
- The DExH/D box protein HEL/UAP56 is essential for mRNA nuclear export in Drosophila.
Gatfield D, Le Hir H, Schmitt C, Braun IC, Köcher T, Wilm M, Izaurralde E. Gatfield D, et al. Curr Biol. 2001 Oct 30;11(21):1716-21. doi: 10.1016/s0960-9822(01)00532-2. Curr Biol. 2001. PMID: 11696332 - Growth-regulated expression and G0-specific turnover of the mRNA that encodes URH49, a mammalian DExH/D box protein that is highly related to the mRNA export protein UAP56.
Pryor A, Tung L, Yang Z, Kapadia F, Chang TH, Johnson LF. Pryor A, et al. Nucleic Acids Res. 2004 Mar 26;32(6):1857-65. doi: 10.1093/nar/gkh347. Print 2004. Nucleic Acids Res. 2004. PMID: 15047853 Free PMC article. - Squid is required for efficient posterior localization of oskar mRNA during Drosophila oogenesis.
Norvell A, Debec A, Finch D, Gibson L, Thoma B. Norvell A, et al. Dev Genes Evol. 2005 Jul;215(7):340-9. doi: 10.1007/s00427-005-0480-2. Epub 2005 Mar 25. Dev Genes Evol. 2005. PMID: 15791421 - RNA localization and translational regulation during axis specification in the Drosophila oocyte.
Cooperstock RL, Lipshitz HD. Cooperstock RL, et al. Int Rev Cytol. 2001;203:541-66. doi: 10.1016/s0074-7696(01)03016-9. Int Rev Cytol. 2001. PMID: 11131526 Review. - Translational regulation and RNA localization in Drosophila oocytes and embryos.
Johnstone O, Lasko P. Johnstone O, et al. Annu Rev Genet. 2001;35:365-406. doi: 10.1146/annurev.genet.35.102401.090756. Annu Rev Genet. 2001. PMID: 11700288 Review.
Cited by
- Prevalent Fast Evolution of Genes Involved in Heterochromatin Functions.
Lin L, Huang Y, McIntyre J, Chang CH, Colmenares S, Lee YCG. Lin L, et al. Mol Biol Evol. 2024 Sep 4;41(9):msae181. doi: 10.1093/molbev/msae181. Mol Biol Evol. 2024. PMID: 39189646 Free PMC article. - An Interaction Network of RNA-Binding Proteins Involved in Drosophila Oogenesis.
Bansal P, Madlung J, Schaaf K, Macek B, Bono F. Bansal P, et al. Mol Cell Proteomics. 2020 Sep;19(9):1485-1502. doi: 10.1074/mcp.RA119.001912. Epub 2020 Jun 17. Mol Cell Proteomics. 2020. PMID: 32554711 Free PMC article. - DDX39B interacts with the pattern recognition receptor pathway to inhibit NF-κB and sensitize to alkylating chemotherapy.
Szymura SJ, Bernal GM, Wu L, Zhang Z, Crawley CD, Voce DJ, Campbell PA, Ranoa DE, Weichselbaum RR, Yamini B. Szymura SJ, et al. BMC Biol. 2020 Mar 24;18(1):32. doi: 10.1186/s12915-020-0764-z. BMC Biol. 2020. PMID: 32209106 Free PMC article. - Su(var)2-10 and the SUMO Pathway Link piRNA-Guided Target Recognition to Chromatin Silencing.
Ninova M, Chen YA, Godneeva B, Rogers AK, Luo Y, Fejes Tóth K, Aravin AA. Ninova M, et al. Mol Cell. 2020 Feb 6;77(3):556-570.e6. doi: 10.1016/j.molcel.2019.11.012. Epub 2019 Dec 31. Mol Cell. 2020. PMID: 31901446 Free PMC article. - UAP56 associates with DRM2 and is localized to chromatin in Arabidopsis.
Azevedo J, Picart C, Dureau L, Pontier D, Jaquinod-Kieffer S, Hakimi MA, Lagrange T. Azevedo J, et al. FEBS Open Bio. 2019 May;9(5):973-985. doi: 10.1002/2211-5463.12627. Epub 2019 Apr 5. FEBS Open Bio. 2019. PMID: 30951268 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases