A systematic library for comprehensive overexpression screens in Saccharomyces cerevisiae - PubMed (original) (raw)
doi: 10.1038/nmeth.1181. Epub 2008 Feb 3.
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- PMID: 18246075
- DOI: 10.1038/nmeth.1181
A systematic library for comprehensive overexpression screens in Saccharomyces cerevisiae
Grace Marie Jones et al. Nat Methods. 2008 Mar.
Abstract
Modern genetic analysis requires the development of new resources to systematically explore gene function in vivo. Overexpression screens are a powerful method to investigate genetic pathways, but the goal of routine and comprehensive overexpression screens has been hampered by the lack of systematic libraries. Here we describe the construction of a systematic collection of the Saccharomyces cerevisiae genome in a high-copy vector and its validation in two overexpression screens.
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