Whole-mount in situ hybridization of small invertebrate embryos using laboratory mini-columns - PubMed (original) (raw)

. 2007 Dec;43(6):764, 766, 768.

doi: 10.2144/000112617.

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• PMID: 18251252
• DOI: 10.2144/000112617

Free article

Whole-mount in situ hybridization of small invertebrate embryos using laboratory mini-columns

Steven Q Irvine. Biotechniques. 2007 Dec.

Free article

Abstract

To facilitate the handling of small invertebrate embryos during whole-mount in situ hybridization, a method of solution exchange using laboratory mini-columns was developed. This protocol speeds time-consuming aspiration of buffers, and avoids accidental loss of the embryos, by gently pushing solutions through the column using air pressure from a syringe after each incubation. The next buffer is then added using a pipettor. Embryos are retained on a filter within the column. As many columns as desired may be processed in parallel for different probes or stages.

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