Human GATA-3: a lineage-restricted transcription factor that regulates the expression of the T cell receptor alpha gene - PubMed (original) (raw)

Human GATA-3: a lineage-restricted transcription factor that regulates the expression of the T cell receptor alpha gene

I C Ho et al. EMBO J. 1991 May.

Abstract

In addition to its role in the recognition of foreign antigens, the T cell receptor (TCR) alpha gene serves as a model system for studies of developmentally-regulated, lineage-specific gene expression in T cells. TCR alpha gene expression is restricted to cells of the TCR alpha/beta+ lineage, and is controlled by a T cell-specific transcriptional enhancer located 4.5 kb 3' to the C alpha gene segment. The TCR alpha enhancer contains four nuclear protein binding sites called T alpha 1-T alpha 4. In this report we describe the identification and characterization of a novel human cDNA, hGATA-3 that binds to the T alpha 3 element of the human TCR alpha enhancer. hGATA-3 contains a zinc finger domain that is highly related to the DNA-binding domain of the erythroid-specific transcription factor, GATA-1, and binds to a region of T alpha 3 that contains a consensus GATA binding site (AGATAG). Northern blot analyses of hematopoietic cell lines demonstrate that hGATA-3 is expressed exclusively in T cells. Overexpression of hGATA-3 in HeLa cells or human B cells specifically activated transcription from a co-transfected reporter plasmid containing two copies of the T alpha 3 binding site located upstream of the minimal SV40 promoter. Taken together these results demonstrate that hGATA-3 is a novel lineage-specific hematopoietic transcription factor that appears to play an important role in regulating the T cell-specific expression of the TCR alpha gene.

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References

    1. Nature. 1988 Dec 8;336(6199):544-51 - PubMed
    1. Eur J Biochem. 1980 Jun;107(2):303-14 - PubMed
    1. Science. 1988 Jul 29;241(4865):577-80 - PubMed
    1. Proc Natl Acad Sci U S A. 1987 May;84(10):3161-5 - PubMed
    1. Proc Natl Acad Sci U S A. 1986 Sep;83(17):6382-6 - PubMed

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