Proteomic analysis of mouse brain microsomes: identification and bioinformatic characterization of endoplasmic reticulum proteins in the mammalian central nervous system - PubMed (original) (raw)

doi: 10.1021/pr7006279. Epub 2008 Feb 14.

Affiliations

PMID: 18271522
DOI: 10.1021/pr7006279

Proteomic analysis of mouse brain microsomes: identification and bioinformatic characterization of endoplasmic reticulum proteins in the mammalian central nervous system

Stanley M Stevens Jr et al. J Proteome Res. 2008 Mar.

Abstract

The endoplasmic reticulum (ER) is the main source for the storage and release of intracellular calcium in neurons and, thus, contributes to the functionality of a diverse set of pathways that control critical aspects of central nervous system function including but not limited to gene expression, neurotransmission, learning, and memory. ER-derived proteins obtained after subcellular fractionation of mouse brain homogenate were digested with trypsin and the corresponding peptides fractionated by strong cation exchange chromatography followed by LC-MS/MS analysis on a hybrid linear ion trap--Fourier transform ion cyclotron resonance (FTICR) mass spectrometer. A comprehensive catalogue representing 1914 proteins was generated from this particular proteomic analysis using identification criteria that corresponded to a false positive identification rate of 0.4%. Various molecular functions and biological processes relevant to the ER were identified upon gene ontology (GO)-based analysis including pathways associated with molecular transport, protein trafficking and localization, and cell signaling. Comparison of the 2D-LC-MS/MS results with those obtained from shotgun LC-MS/MS analyses demonstrated that most molecular functions and biological processes were represented via GO analysis using either methodology. Results from this comparison as well as a focused investigation into components of calcium-mediated signaling in the mouse brain ER are also presented.

PubMed Disclaimer

Cited by

Water-soluble mmp-9 inhibitor prodrug generates active metabolites that cross the blood-brain barrier.
Song W, Peng Z, Gooyit M, Suckow MA, Schroeder VA, Wolter WR, Lee M, Ikejiri M, Cui J, Gu Z, Chang M. Song W, et al. ACS Chem Neurosci. 2013 Aug 21;4(8):1168-73. doi: 10.1021/cn400077d. Epub 2013 May 30. ACS Chem Neurosci. 2013. PMID: 23687970 Free PMC article.
Group 1 metabotropic glutamate receptors 1 and 5 form a protein complex in mouse hippocampus and cortex.
Pandya NJ, Klaassen RV, van der Schors RC, Slotman JA, Houtsmuller A, Smit AB, Li KW. Pandya NJ, et al. Proteomics. 2016 Oct;16(20):2698-2705. doi: 10.1002/pmic.201500400. Epub 2016 Sep 12. Proteomics. 2016. PMID: 27392515 Free PMC article.
Proteomic analysis of signaling network regulation in renal cell carcinomas with differential hypoxia-inducible factor-2α expression.
Nagaprashantha LD, Talamantes T, Singhal J, Guo J, Vatsyayan R, Rauniyar N, Awasthi S, Singhal SS, Prokai L. Nagaprashantha LD, et al. PLoS One. 2013 Aug 5;8(8):e71654. doi: 10.1371/journal.pone.0071654. Print 2013. PLoS One. 2013. PMID: 23940778 Free PMC article.
Mandatory role of endoplasmic reticulum and its pentose phosphate shunt in the myocardial defense mechanisms against the redox stress induced by anthracyclines.
Sambuceti G, Cossu V, Vitale F, Bianconi E, Carta S, Venturi C, Chiesa S, Lanfranchi F, Emionite L, Carlone S, Sofia L, D'Amico F, Di Raimondo T, Chiola S, Orengo AM, Morbelli S, Ameri P, Bauckneht M, Marini C. Sambuceti G, et al. Mol Cell Biochem. 2024 Nov;479(11):2973-2987. doi: 10.1007/s11010-023-04903-z. Epub 2023 Dec 12. Mol Cell Biochem. 2024. PMID: 38082185 Free PMC article.
The contribution of systematic approaches to characterizing the proteins and functions of the endoplasmic reticulum.
Schuldiner M, Weissman JS. Schuldiner M, et al. Cold Spring Harb Perspect Biol. 2013 Mar 1;5(3):a013284. doi: 10.1101/cshperspect.a013284. Cold Spring Harb Perspect Biol. 2013. PMID: 23359093 Free PMC article. Review.

Proteomic analysis of mouse brain microsomes: identification and bioinformatic characterization of endoplasmic reticulum proteins in the mammalian central nervous system - PubMed (original) (raw)