Attenuated vancomycin bactericidal activity against Staphylococcus aureus hemB mutants expressing the small-colony-variant phenotype - PubMed (original) (raw)

Attenuated vancomycin bactericidal activity against Staphylococcus aureus hemB mutants expressing the small-colony-variant phenotype

Brian T Tsuji et al. Antimicrob Agents Chemother. 2008 Apr.

Abstract

The in vitro bactericidal activities of vancomycin against Staphylococcus aureus hemB mutants displaying the small-colony-variant phenotype and their parental strains were evaluated. Vancomycin killing activities against hemB mutants were markedly attenuated, demonstrating approximately 50% less effect, a result which was well described by a Hill-type pharmacodynamic model.

PubMed Disclaimer

Figures

FIG. 1.

Results from vancomycin time-kill experiments evaluating bactericidal activity toward S. aureus strains displaying the normal (N) and SCV phenotypes. The key to symbols for each regimen is as follows: control (•) and 0.25 (▾), 0.5 (▪), 1 (♦), 2 (▴), 4 ( formula image ), 8 (•), 16 (▾), 32 (▪), and 64 (♦) times the MIC. MSSA, methicillin-susceptible S. aureus.

Cited by

Activity of AFN-1252, a novel FabI inhibitor, against Staphylococcus aureus in an in vitro pharmacodynamic model simulating human pharmacokinetics.
Tsuji BT, Harigaya Y, Lesse AJ, Forrest A, Ngo D. Tsuji BT, et al. J Chemother. 2013 Feb;25(1):32-5. doi: 10.1179/1973947812Y.0000000060. J Chemother. 2013. PMID: 23433442 Free PMC article.
Azithromycin Pharmacodynamics against Persistent Haemophilus influenzae in Chronic Obstructive Pulmonary Disease.
Tsuji BT, Fisher J, Boadi-Yeboah R, Holden PN, Sethi S, Pettigrew MM, Murphy TF. Tsuji BT, et al. Antimicrob Agents Chemother. 2018 Jan 25;62(2):e01995-17. doi: 10.1128/AAC.01995-17. Print 2018 Feb. Antimicrob Agents Chemother. 2018. PMID: 29180527 Free PMC article.
Paradoxical Effect of Polymyxin B: High Drug Exposure Amplifies Resistance in Acinetobacter baumannii.
Tsuji BT, Landersdorfer CB, Lenhard JR, Cheah SE, Thamlikitkul V, Rao GG, Holden PN, Forrest A, Bulitta JB, Nation RL, Li J. Tsuji BT, et al. Antimicrob Agents Chemother. 2016 Jun 20;60(7):3913-20. doi: 10.1128/AAC.02831-15. Print 2016 Jul. Antimicrob Agents Chemother. 2016. PMID: 27067330 Free PMC article.
Comparative pharmacodynamics of four different carbapenems in combination with polymyxin B against carbapenem-resistant Acinetobacter baumannii.
Lenhard JR, Gall JS, Bulitta JB, Thamlikitkul V, Landersdorfer CB, Forrest A, Nation RL, Li J, Tsuji BT. Lenhard JR, et al. Int J Antimicrob Agents. 2016 Dec;48(6):719-724. doi: 10.1016/j.ijantimicag.2016.07.024. Epub 2016 Sep 20. Int J Antimicrob Agents. 2016. PMID: 27773498 Free PMC article.
Combinatorial Pharmacodynamics of Ceftolozane-Tazobactam against Genotypically Defined β-Lactamase-Producing Escherichia coli: Insights into the Pharmacokinetics/Pharmacodynamics of β-Lactam-β-Lactamase Inhibitor Combinations.
Soon RL, Lenhard JR, Bulman ZP, Holden PN, Kelchlin P, Steenbergen JN, Friedrich LV, Forrest A, Tsuji BT. Soon RL, et al. Antimicrob Agents Chemother. 2016 Mar 25;60(4):1967-73. doi: 10.1128/AAC.02635-15. Print 2016 Apr. Antimicrob Agents Chemother. 2016. PMID: 26729494 Free PMC article.

References

1. Baumert, N., C. von Eiff, F. Schaaff, G. Peters, R. A. Proctor, and H. G. Sahl. 2002. Physiology and antibiotic susceptibility of Staphylococcus aureus small colony variants. Microb. Drug Resist. 8:253-260. - PubMed
1. Bayston, R., W. Ashraf, and T. Smith. 2007. Triclosan resistance in methicillin-resistant Staphylococcus aureus expressed as small colony variants: a novel mode of evasion of susceptibility to antiseptics. J. Antimicrob. Chemother. 60:176-177. - PubMed
1. Bischoff, M., J. M. Entenza, and P. Giachino. 2001. Influence of a functional sigB operon on the global regulators sar and agr in Staphylococcus aureus. J. Bacteriol. 183:5171-5179. - PMC - PubMed
1. Brouillette, E., A. Martinez, B. J. Boyll, N. E. Allen, and F. Malouin. 2004. Persistence of a Staphylococcus aureus small-colony variant under antibiotic pressure in vivo. FEMS Immunol. Med. Microbiol. 41:35-41. - PubMed
1. Chuard, C., P. E. Vaudaux, R. A. Proctor, and D. P. Lew. 1997. Decreased susceptibility to antibiotic killing of a stable small colony variant of Staphylococcus aureus in fluid phase and on fibronectin-coated surfaces. J. Antimicrob. Chemother. 39:603-608. - PubMed

Attenuated vancomycin bactericidal activity against Staphylococcus aureus hemB mutants expressing the small-colony-variant phenotype - PubMed (original) (raw)