Plasmid-mediated qepA gene among Escherichia coli clinical isolates from Japan - PubMed (original) (raw)

Plasmid-mediated qepA gene among Escherichia coli clinical isolates from Japan

Kunikazu Yamane et al. Antimicrob Agents Chemother. 2008 Apr.

Abstract

Seven hundred fifty-one Escherichia coli clinical isolates collected from 140 Japanese hospitals between 2002 and 2006 were screened for the qepA and qnr genes. Two E. coli isolates (0.3%) harbored qepA, but no qnr was identified. The results suggested a low prevalence of E. coli harboring qepA or qnr in Japan.

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Figures

FIG. 1.

(A) EcoRI restriction profiles of _qepA_-carrying plasmids from the transconjugants. Lane M, lambda HindIII marker; lane 1, pHPA from E. coli C316; lane 2, p041060 from E. coli MRY04-1060; lane 3, p05283 from E. coli MRY05-3283. (B) PFGE fingerprinting patterns of XbaI-digested total DNA preparations from three E. coli isolates. Lanes M, lambda ladder PFGE marker used as a molecular size marker. Lanes 1 to 3, E. coli C316, MRY04-1030, and MRY05-3283, respectively.

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