Resolution of Holliday junctions in vitro requires the Escherichia coli ruvC gene product - PubMed (original) (raw)
Resolution of Holliday junctions in vitro requires the Escherichia coli ruvC gene product
B Connolly et al. Proc Natl Acad Sci U S A. 1991.
Abstract
In previous studies, Holliday junctions generated during RecA-mediated strand-exchange reactions were resolved by fractionated Escherichia coli extracts. We now report the specific binding and cleavage of synthetic Holliday junctions (50 base pairs long) by a fraction purified by chromatography on DEAE-cellulose, phosphocellulose, and single-stranded DNA-cellulose. The cleavage reaction provided a sensitive assay with which to screen extracts prepared from recombination/repair-deficient mutants. Cells with mutations in ruvC lack the nuclease activity that cleaves synthetic Holliday junctions in vitro. This deficiency was restored by a multicopy plasmid carrying a ruvC+ gene that overexpressed junction-resolving activity. The UV sensitivity and deficiency in recombinational repair of DNA exhibited by ruv mutants lead us to suggest that RuvC resolves Holliday junctions in vivo.
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References
- Mol Gen Genet. 1991 Feb;225(2):266-72 - PubMed
- J Bacteriol. 1974 Feb;117(2):337-44 - PubMed
- J Bacteriol. 1990 Nov;172(11):6291-9 - PubMed
- J Bacteriol. 1985 Sep;163(3):1060-6 - PubMed
- J Bacteriol. 1988 Sep;170(9):4322-9 - PubMed
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