Localization of pre-messenger RNA at discrete nuclear sites - PubMed (original) (raw)

Localization of pre-messenger RNA at discrete nuclear sites

J Wang et al. Proc Natl Acad Sci U S A. 1991.

Abstract

We have studied the nuclear localization of rhodamine-labeled pre-mRNA after microinjection into nuclei of cultured rat kidney epithelial cells. Intranuclear localization of the injected RNA was followed in the living cells by fluorescence microscopy and digital image processing. Injected human beta-globin pre-mRNA became localized in 30-60 discrete nuclear sites that were coincident with loci defined by monoclonal antibodies against small nuclear ribonucleoproteins (Sm) or another spliceosome component (SC-35) in parallel immunocytochemical studies on the same nuclei. Similar patterns of nuclear localization were observed with a rat proenkephalin pre-mRNA. Nuclear microinjection of an intronlacking beta-globin RNA, a splicing-defective beta-globin mutant pre-mRNA, or an antisense beta-globin pre-mRNA did not result in localization at discrete sites. These results indicate that pre-mRNA binds preferentially to nuclear Sm and SC-35 antibody-reactive sites in vivo and that the binding requires intron sequences.

PubMed Disclaimer

Similar articles

• Spliceosome assembly involves the binding and release of U4 small nuclear ribonucleoprotein.
  Lamond AI, Konarska MM, Grabowski PJ, Sharp PA. Lamond AI, et al. Proc Natl Acad Sci U S A. 1988 Jan;85(2):411-5. doi: 10.1073/pnas.85.2.411. Proc Natl Acad Sci U S A. 1988. PMID: 2963332 Free PMC article.
• Intron-dependent recruitment of pre-mRNA splicing factors to sites of transcription.
  Huang S, Spector DL. Huang S, et al. J Cell Biol. 1996 May;133(4):719-32. doi: 10.1083/jcb.133.4.719. J Cell Biol. 1996. PMID: 8666659 Free PMC article.
• Intron sequences and the length of the downstream second exon affect the binding of hnRNP C proteins in an in vitro splicing reaction.
  Goswami P, Goldenberg CJ. Goswami P, et al. Nucleic Acids Res. 1988 Jun 10;16(11):4995-5011. doi: 10.1093/nar/16.11.4995. Nucleic Acids Res. 1988. PMID: 3290845 Free PMC article.
• Mechanisms and Regulation of Alternative Pre-mRNA Splicing.
  Lee Y, Rio DC. Lee Y, et al. Annu Rev Biochem. 2015;84:291-323. doi: 10.1146/annurev-biochem-060614-034316. Epub 2015 Mar 12. Annu Rev Biochem. 2015. PMID: 25784052 Free PMC article. Review.
• Nuclear RNA processing.
  Lamond AI. Lamond AI. Curr Opin Cell Biol. 1991 Jun;3(3):493-501. doi: 10.1016/0955-0674(91)90078-d. Curr Opin Cell Biol. 1991. PMID: 1716447 Review.

Cited by

• Post-transcriptional spliceosomes are retained in nuclear speckles until splicing completion.
  Girard C, Will CL, Peng J, Makarov EM, Kastner B, Lemm I, Urlaub H, Hartmuth K, Lührmann R. Girard C, et al. Nat Commun. 2012;3:994. doi: 10.1038/ncomms1998. Nat Commun. 2012. PMID: 22871813
• U1 and U2 small nuclear RNAs are present in nuclear speckles.
  Huang S, Spector DL. Huang S, et al. Proc Natl Acad Sci U S A. 1992 Jan 1;89(1):305-8. doi: 10.1073/pnas.89.1.305. Proc Natl Acad Sci U S A. 1992. PMID: 1530887 Free PMC article.
• Fluorescent RNA cytochemistry: tracking gene transcripts in living cells.
  Pederson T. Pederson T. Nucleic Acids Res. 2001 Mar 1;29(5):1013-6. doi: 10.1093/nar/29.5.1013. Nucleic Acids Res. 2001. PMID: 11222750 Free PMC article. Review.
• The nucleolus and the four ribonucleoproteins of translation.
  Pederson T, Politz JC. Pederson T, et al. J Cell Biol. 2000 Mar 20;148(6):1091-5. doi: 10.1083/jcb.148.6.1091. J Cell Biol. 2000. PMID: 10725320 Free PMC article. Review. No abstract available.
• Nucleolar localization elements of Xenopus laevis U3 small nucleolar RNA.
  Lange TS, Ezrokhi M, Borovjagin AV, Rivera-León R, North MT, Gerbi SA. Lange TS, et al. Mol Biol Cell. 1998 Oct;9(10):2973-85. doi: 10.1091/mbc.9.10.2973. Mol Biol Cell. 1998. PMID: 9763456 Free PMC article.

References

1. 1. J Cell Biol. 1986 Oct;103(4):1153-7 - PubMed
2. 1. J Cell Biol. 1984 Jan;98(1):358-63 - PubMed
3. 1. Nucleic Acids Res. 1989 Aug 25;17(16):6553-68 - PubMed
4. 1. Proc Natl Acad Sci U S A. 1989 Sep;86(17):6635-9 - PubMed
5. 1. Exp Cell Res. 1984 Oct;154(2):548-60 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central

• Other Literature Sources

  • The Lens - Patent Citations Database