The phosphorylated form of the enhancer-binding protein NTRC has an ATPase activity that is essential for activation of transcription - PubMed (original) (raw)
The phosphorylated form of the enhancer-binding protein NTRC has an ATPase activity that is essential for activation of transcription
D S Weiss et al. Cell. 1991.
Abstract
The NTRC protein of enteric bacteria is an enhancer-binding protein that activates transcription in response to limitation of combined nitrogen. NTRC activates transcription by catalyzing formation of open complexes by RNA polymerase (sigma 54 holoenzyme form) in an ATP-dependent reaction. To catalyze open complex formation, NTRC must be phosphorylated. We show that phosphorylated NTRC has an ATPase activity, and we present biochemical and genetic evidence that NTRC must hydrolyze ATP to catalyze open complex formation. It is likely that all activators of sigma 54 holoenzyme have an ATPase activity.
Similar articles
- Repressor forms of the enhancer-binding protein NrtC: some fail in coupling ATP hydrolysis to open complex formation by sigma 54-holoenzyme.
North AK, Weiss DS, Suzuki H, Flashner Y, Kustu S. North AK, et al. J Mol Biol. 1996 Jul 19;260(3):317-31. doi: 10.1006/jmbi.1996.0403. J Mol Biol. 1996. PMID: 8757796 - The prokaryotic enhancer binding protein NTRC has an ATPase activity which is phosphorylation and DNA dependent.
Austin S, Dixon R. Austin S, et al. EMBO J. 1992 Jun;11(6):2219-28. doi: 10.1002/j.1460-2075.1992.tb05281.x. EMBO J. 1992. PMID: 1534752 Free PMC article. - Glutamate at the site of phosphorylation of nitrogen-regulatory protein NTRC mimics aspartyl-phosphate and activates the protein.
Klose KE, Weiss DS, Kustu S. Klose KE, et al. J Mol Biol. 1993 Jul 5;232(1):67-78. doi: 10.1006/jmbi.1993.1370. J Mol Biol. 1993. PMID: 8331671 - The bacterial enhancer-binding protein NtrC as a molecular machine.
Rombel I, North A, Hwang I, Wyman C, Kustu S. Rombel I, et al. Cold Spring Harb Symp Quant Biol. 1998;63:157-66. doi: 10.1101/sqb.1998.63.157. Cold Spring Harb Symp Quant Biol. 1998. PMID: 10384279 Review. No abstract available. - Regulation of transcription of the glnALG operon of Escherichia coli by protein phosphorylation.
Magasanik B. Magasanik B. Biochimie. 1989 Sep-Oct;71(9-10):1005-12. doi: 10.1016/0300-9084(89)90104-1. Biochimie. 1989. PMID: 2574599 Review.
Cited by
- Nitrogen-Fixing Gamma Proteobacteria _Azotobacter vinelandii_-A Blueprint for Nitrogen-Fixing Plants?
Barron S, Mus F, Peters JW. Barron S, et al. Microorganisms. 2024 Oct 18;12(10):2087. doi: 10.3390/microorganisms12102087. Microorganisms. 2024. PMID: 39458396 Free PMC article. Review. - Transcription factor-based biosensors enlightened by the analyte.
Fernandez-López R, Ruiz R, de la Cruz F, Moncalián G. Fernandez-López R, et al. Front Microbiol. 2015 Jul 1;6:648. doi: 10.3389/fmicb.2015.00648. eCollection 2015. Front Microbiol. 2015. PMID: 26191047 Free PMC article. Review. - Free energy landscape of activation in a signalling protein at atomic resolution.
Pontiggia F, Pachov DV, Clarkson MW, Villali J, Hagan MF, Pande VS, Kern D. Pontiggia F, et al. Nat Commun. 2015 Jun 15;6:7284. doi: 10.1038/ncomms8284. Nat Commun. 2015. PMID: 26073309 Free PMC article. - Phosphorylation site of NtrC, a protein phosphatase whose covalent intermediate activates transcription.
Sanders DA, Gillece-Castro BL, Burlingame AL, Koshland DE Jr. Sanders DA, et al. J Bacteriol. 1992 Aug;174(15):5117-22. doi: 10.1128/jb.174.15.5117-5122.1992. J Bacteriol. 1992. PMID: 1321122 Free PMC article. - P(II) signal transduction proteins, pivotal players in microbial nitrogen control.
Arcondéguy T, Jack R, Merrick M. Arcondéguy T, et al. Microbiol Mol Biol Rev. 2001 Mar;65(1):80-105. doi: 10.1128/MMBR.65.1.80-105.2001. Microbiol Mol Biol Rev. 2001. PMID: 11238986 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases