Synthesis of new fluorogenic cyanine dyes and incorporation into RNA fluoromodules - PubMed (original) (raw)
. 2008 Apr 17;10(8):1561-4.
doi: 10.1021/ol702920e. Epub 2008 Mar 14.
Affiliations
- PMID: 18338898
- DOI: 10.1021/ol702920e
Synthesis of new fluorogenic cyanine dyes and incorporation into RNA fluoromodules
Tudor P Constantin et al. Org Lett. 2008.
Abstract
A new fluorogenic cyanine dye was synthesized and found to have low fluorescence quantum yield in fluid solution and in the presence of double-stranded DNA but 80-fold enhanced fluorescence in viscous glycerol solution. An RNA aptamer selected for binding to the new dye exhibits K(d) = 87 nM and 60-fold fluorescence enhancement. The dye-aptamer pair is a fluoromodule that can be incorporated into fluorescent sensors and labels.
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