Ectopic expression of eIF4E-transporter triggers the movement of eIF4E into P-bodies, inhibiting steady-state translation but not the pioneer round of translation - PubMed (original) (raw)
. 2008 May 16;369(4):1160-5.
doi: 10.1016/j.bbrc.2008.03.017. Epub 2008 Mar 14.
Affiliations
- PMID: 18343217
- DOI: 10.1016/j.bbrc.2008.03.017
Ectopic expression of eIF4E-transporter triggers the movement of eIF4E into P-bodies, inhibiting steady-state translation but not the pioneer round of translation
Hyung Chul Lee et al. Biochem Biophys Res Commun. 2008.
Abstract
Nonsense-mediated mRNA decay (NMD) is the best-characterized mRNA surveillance mechanism; this process removes faulty mRNAs harboring premature termination codons (PTCs). NMD targets newly synthesized mRNAs bound by nuclear cap-binding proteins 80/20 (CBP80/20) and exon junction complex (EJC), the former of which is thought to recruit the ribosome to initiate the pioneer round of translation. After completion of the pioneer round of translation, CBP80/20 is replaced by the cytoplasmic cap-binding protein eIF4E, which mediates steady-state translation in the cytoplasm. Here, we show that overexpression of eIF4E-T preferentially inhibits cap-dependent steady-state translation, but not the pioneer round of translation. We also demonstrate that overexpression of eIF4E-T or Dcp1a triggers the movement of eIF4E into the processing bodies. These results suggest that the pioneer round of translation differs from steady-state translation in terms of ribosome recruitment.
Similar articles
- Pioneer round of translation mediated by nuclear cap-binding proteins CBP80/20 occurs during prolonged hypoxia.
Oh N, Kim KM, Choe J, Kim YK. Oh N, et al. FEBS Lett. 2007 Oct 30;581(26):5158-64. doi: 10.1016/j.febslet.2007.10.002. Epub 2007 Oct 11. FEBS Lett. 2007. PMID: 17942097 - The pioneer translation initiation complex is functionally distinct from but structurally overlaps with the steady-state translation initiation complex.
Chiu SY, Lejeune F, Ranganathan AC, Maquat LE. Chiu SY, et al. Genes Dev. 2004 Apr 1;18(7):745-54. doi: 10.1101/gad.1170204. Epub 2004 Apr 1. Genes Dev. 2004. PMID: 15059963 Free PMC article. - Failsafe nonsense-mediated mRNA decay does not detectably target eIF4E-bound mRNA.
Matsuda D, Hosoda N, Kim YK, Maquat LE. Matsuda D, et al. Nat Struct Mol Biol. 2007 Oct;14(10):974-9. doi: 10.1038/nsmb1297. Epub 2007 Sep 16. Nat Struct Mol Biol. 2007. PMID: 17873884 - Regulation of cap-dependent translation by eIF4E inhibitory proteins.
Richter JD, Sonenberg N. Richter JD, et al. Nature. 2005 Feb 3;433(7025):477-80. doi: 10.1038/nature03205. Nature. 2005. PMID: 15690031 Review. - CBP80-promoted mRNP rearrangements during the pioneer round of translation, nonsense-mediated mRNA decay, and thereafter.
Maquat LE, Hwang J, Sato H, Tang Y. Maquat LE, et al. Cold Spring Harb Symp Quant Biol. 2010;75:127-34. doi: 10.1101/sqb.2010.75.028. Epub 2011 Mar 29. Cold Spring Harb Symp Quant Biol. 2010. PMID: 21447822 Free PMC article. Review.
Cited by
- Optimization of immunoprecipitation-western blot analysis in detecting GW182-associated components of GW/P bodies.
Moser JJ, Chan EK, Fritzler MJ. Moser JJ, et al. Nat Protoc. 2009;4(5):674-85. doi: 10.1038/nprot.2009.34. Nat Protoc. 2009. PMID: 19373232 Free PMC article. - The pioneer round of translation: features and functions.
Maquat LE, Tarn WY, Isken O. Maquat LE, et al. Cell. 2010 Aug 6;142(3):368-74. doi: 10.1016/j.cell.2010.07.022. Cell. 2010. PMID: 20691898 Free PMC article. - Targeting a ribonucleoprotein complex containing the caprin-1 protein and the c-Myc mRNA suppresses tumor growth in mice: an identification of a novel oncotarget.
Qiu YQ, Yang CW, Lee YZ, Yang RB, Lee CH, Hsu HY, Chang CC, Lee SJ. Qiu YQ, et al. Oncotarget. 2015 Feb 10;6(4):2148-63. doi: 10.18632/oncotarget.3236. Oncotarget. 2015. PMID: 25669982 Free PMC article. - The DDX6-4E-T interaction mediates translational repression and P-body assembly.
Kamenska A, Simpson C, Vindry C, Broomhead H, Bénard M, Ernoult-Lange M, Lee BP, Harries LW, Weil D, Standart N. Kamenska A, et al. Nucleic Acids Res. 2016 Jul 27;44(13):6318-34. doi: 10.1093/nar/gkw565. Epub 2016 Jun 24. Nucleic Acids Res. 2016. PMID: 27342281 Free PMC article. - Distinct recruitment of human eIF4E isoforms to processing bodies and stress granules.
Frydryskova K, Masek T, Borcin K, Mrvova S, Venturi V, Pospisek M. Frydryskova K, et al. BMC Mol Biol. 2016 Aug 30;17(1):21. doi: 10.1186/s12867-016-0072-x. BMC Mol Biol. 2016. PMID: 27578149 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous