Transforming growth factor-beta-stimulated endocardial cell transformation is dependent on Par6c regulation of RhoA - PubMed (original) (raw)

ROCK1/2 inhibition via Y27632 leads to ventricular EMT. A and B, data points are mean number of cells invading the collagen gel, derived from three independent experiments normalized to vehicle. A, Y27632 does not significantly block AVC transformation when used near the published IC50 valve of 0.22 μ

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, but does significantly reduce EMT at high concentrations. Vehicle (DMSO): normalized to 100%; 0.3 μ

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: 89 ± 4.9%; 3.0 μ

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: 78 ± 4.7%; 30 μ

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: 60 ± 10.4%; 60 μ

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: 40 ± 5.0%. The number of AVC explants examined were as follows: vehicle (n = 23; total number of invaded cells, 3186), n = number of explants, 0.3 μ

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(n = 25; total number of invaded cells, 3087), 3.0 μ

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(n = 26; total number of invaded cells, 2831), 30 μ

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(n = 33; total number of invaded cells, 2751), 60 μ

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(n = 24; total number of invaded cells, 1318). We observe a linear decrease in cell motility above 0.5 μ

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, suggestive of nonspecific effects on cell viability at higher concentrations. B, Y27632 leads to EMT in ventricular explants. Vehicle (DMSO): normalized to 100%. 0.5 μ

m

: 368 ± 23%; 30 μ

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: 92 ± 11%. The numbers of ventricular explants examined were as follows: vehicle (n = 30; total number of invaded cells, 219), n = number of explants, 0.5 μ

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(n = 30; total number of invaded cells, 800), 30 μ

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(n = 30; total number of invaded cells, 199).