Assessment of a non-invasive high-throughput classifier for behaviours associated with sleep and wake in mice - PubMed (original) (raw)

Assessment of a non-invasive high-throughput classifier for behaviours associated with sleep and wake in mice

Kevin D Donohue et al. Biomed Eng Online. 2008.

Abstract

This work presents a non-invasive high-throughput system for automatically detecting characteristic behaviours in mice over extended periods of time, useful for phenotyping experiments. The system classifies time intervals on the order of 2 to 4 seconds as corresponding to motions consistent with either active wake or inactivity associated with sleep. A single Polyvinylidine Difluoride (PVDF) sensor on the cage floor generates signals from motion resulting in pressure. This paper develops a linear classifier based on robust features extracted from normalized power spectra and autocorrelation functions, as well as novel features from the collapsed average (autocorrelation of complex spectrum), which characterize transient and periodic properties of the signal envelope. Performance is analyzed through an experiment comparing results from direct human observation and classification of the different behaviours with an automatic classifier used in conjunction with this system. Experimental results from over 28.5 hours of data from 4 mice indicate a 94% classification rate relative to the human observations. Examples of sequential classifications (2 second increments) over transition regions between sleep and wake behaviour are also presented to demonstrate robust performance to signal variation and explain performance limitations.

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Figures

Figure 1

Figure 1

Quad cage and sensor system (a) top view showing cage walls on top of sensors on base (b) side view showing sensor layers on cage floor and connection to sensor amplifier.

Figure 2

Figure 2

Example of piezoelectric signals corresponding to sleep from 2 different mice showing quasi-periodicity with (a) High-amplitude and (b) Low-amplitude.

Figure 3

Figure 3

Examples of piezoelectric signals corresponding to wake from the same mouse showing random transient-like signals corresponding to (a) quite rest, and (b) motion across the cage floor.

Figure 4

Figure 4

Examples of (a) PS for sleep signals of Fig. 1,(b) PS for wake signals of Fig. 2,(c) AC for sleep signals of Fig. 1,(d) AC for wake signals of Fig. 2,(e) CA for sleep signals of Fig. 1,(f) CA for wake signals of Fig. 2.

Figure 5

Figure 5

Sensor signal with corresponding sleep-wake decision statistic computed every 2 seconds. Long time-range to observe large scale signals behaviour over sleep and active periods with corresponding decision statistics

Figure 6

Figure 6

Sensor signal with corresponding sleep-wake decision statistic computed every 2 seconds. Long time-range to observe gross signal behaviour over sleep and active periods with corresponding decision statistics.

Figure 7

Figure 7

Sensor signal with corresponding sleep-wake decision statistic computed every 2 seconds indicate by X markers. Time series shows transition between rest and sleep and response of classifier decision statistics.

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References

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