Plasmacytoid dendritic cells migrate in afferent skin lymph - PubMed (original) (raw)

. 2008 May 1;180(9):5963-72.

doi: 10.4049/jimmunol.180.9.5963.

Vanessa Contreras, Michel Bonneau, Alexandre Courbet, Stefan Chilmonczyk, Claudia Bevilacqua, Mathieu Epardaud, Violeta Niborski, Sabine Riffault, Anne-Marie Balazuc, Eliane Foulon, Laurence Guzylack-Piriou, Beatrice Riteau, Jayne Hope, Nicolas Bertho, Bernard Charley, Isabelle Schwartz-Cornil

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Plasmacytoid dendritic cells migrate in afferent skin lymph

Florentina Pascale et al. J Immunol. 2008.

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Abstract

Conventional dendritic cells enter lymph nodes by migrating from peripheral tissues via the lymphatic route, whereas plasmacytoid dendritic cells (pDC), also called IFN-producing cells (IPC), are described to gain nodes from blood via the high endothelial venules. We demonstrate here that IPC/pDC migrate in the afferent lymph of two large mammals. In sheep, injection of type A CpG oligodinucleotide (ODN) induced lymph cells to produce type I IFN. Furthermore, low-density lymph cells collected at steady state produced type I IFN after stimulation with type A CpG ODN and enveloped viruses. Sheep lymph IPC were found within a minor B(neg)CD11c(neg) subset expressing CD45RB. They presented a plasmacytoid morphology, expressed high levels of TLR-7, TLR-9, and IFN regulatory factor 7 mRNA, induced IFN-gamma production in allogeneic CD4(pos) T cells, and differentiated into dendritic cell-like cells under viral stimulation, thus fulfilling criteria of bona fide pDC. In mini-pig, a CD4(pos)SIRP(pos) subset in afferent lymph cells, corresponding to pDC homologs, produced type I IFN after type A CpG-ODN triggering. Thus, pDC can link innate and acquired immunity by migrating from tissue to draining node via lymph, similarly to conventional dendritic cells.

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