Identificating cathepsin D as a biomarker for differentiation and prognosis of nasopharyngeal carcinoma by laser capture microdissection and proteomic analysis - PubMed (original) (raw)

doi: 10.1021/pr7008548. Epub 2008 Apr 24.

Wei-Guo Huang, Zhu-Chu Chen, Peng-Fei Zhang, Mao-Yu Li, Feng Li, Jian-Ling Li, Cui Li, Hong Yi, Fang Peng, Chao-Jun Duan, Zhi-Qiang Xiao

Affiliations

• PMID: 18433155
• DOI: 10.1021/pr7008548

Identificating cathepsin D as a biomarker for differentiation and prognosis of nasopharyngeal carcinoma by laser capture microdissection and proteomic analysis

Ai-Lan Cheng et al. J Proteome Res. 2008 Jun.

Abstract

In this study, we applied laser capture microdissection and a proteomic approach to identify novel nasopharyngeal carcinoma (NPC) biomarkers. Proteins from pooled microdissected NPC and normal nasopharyngeal epithelial tissues (NNET) were separated by two-dimensional gel electrophoresis, and differential proteins were identified by mass spectrometry. Expression of the differential protein cathepsin D in the above two tissues as well as four NPC cell lines was determined by Western blotting. Next, siRNA was used to inhibit the expression of cathepsin D in highly metastatic NPC cell line 5-8F to examine whether it associates with NPC metastasis. Immunohistochemistry was also performed to detect the expression of cathepsin D in 72 cases of primary NPC, 28 cases of NNET, and 20 cases of cervical lymph node metastases, and the correlation of its expression level with clinicopathologic features and clinical outcomes were evaluated. Thirty-six differential proteins between the NPC and NNET were identified. The expression level of cathepsin D in the two types of tissues was confirmed by Western blotting and related to differentiation degree and metastatic potential of the NPC cell lines. Down-regulated cathepsin D expression by siRNA significantly decreased in vitro invasive ability of 5-8F cells. Significant cathepsin D down-regulation was observed in NPC versus NNET, whereas significant cathepsin D up-regulation was observed in lymph node metastasis versus primary NPC. In addition, cathepsin D down-regulation was significantly correlated with poor histological differentiation, whereas cathepsin D up-regulation was significantly correlated with advanced clinical stage, recurrence, and lymph node and distant metastasis. Furthermore, survival curves showed that patients with cathepsin D up-regulation had a poor prognosis. Multivariate analysis confirmed that cathepsin D expression was an independent prognostic indicator. The data suggest that cathepsin D is a potential biomarker for the differentiation and prognosis of NPC, and its dysregulation might play an important role in the pathogenesis of NPC.

PubMed Disclaimer

Similar articles

• Identification of novel nasopharyngeal carcinoma biomarkers by laser capture microdissection and proteomic analysis.
  Cheng AL, Huang WG, Chen ZC, Peng F, Zhang PF, Li MY, Li F, Li JL, Li C, Yi H, Yi B, Xiao ZQ. Cheng AL, et al. Clin Cancer Res. 2008 Jan 15;14(2):435-45. doi: 10.1158/1078-0432.CCR-07-1215. Clin Cancer Res. 2008. PMID: 18223218
• Proteomic analysis to identify cytokeratin 18 as a novel biomarker of nasopharyngeal carcinoma.
  Li XM, Huang WG, Yi H, Cheng AL, Xiao ZQ. Li XM, et al. J Cancer Res Clin Oncol. 2009 Dec;135(12):1763-75. doi: 10.1007/s00432-009-0623-3. Epub 2009 Jun 16. J Cancer Res Clin Oncol. 2009. PMID: 19529960
• [Screening for differentially expressed proteins in nasopharyngeal carcinoma by laser capture microdissection and proteomic analysis].
  Yi H, Cheng AL, Huang WG, Zhang PF, Li MY, Peng F, Li F, Li C, Chen ZC, Xiao ZQ. Yi H, et al. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2008 May;33(5):375-83. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2008. PMID: 18544839 Chinese.
• Needle in a haystack: microdissecting the proteome of a tissue.
  Ball HJ, Hunt NH. Ball HJ, et al. Amino Acids. 2004 Aug;27(1):1-7. doi: 10.1007/s00726-004-0104-6. Epub 2004 Jul 14. Amino Acids. 2004. PMID: 15309566 Review.
• [Mechanism of the overexpression of the cathepsin D gene in breast cancer and consequences in the metastatic process].
  Rochefort H. Rochefort H. C R Seances Soc Biol Fil. 1991;185(6):415-21. C R Seances Soc Biol Fil. 1991. PMID: 1822391 Review. French.

Cited by

• Identification of RKIP as a differentially tyrosine-phosphorylated protein in nasopharyngeal carcinoma and normal nasopharyngeal epithelial tissues by phosphoproteomic approach.
  Chen Y, Tang CE, Ouyang GL, Ruan L, Li MY, Zhang PF, Li C, Yi H, Peng F, Li JL, Chen ZC, Xiao ZQ. Chen Y, et al. Med Oncol. 2009 Dec;26(4):463-70. doi: 10.1007/s12032-008-9147-y. Epub 2008 Dec 19. Med Oncol. 2009. PMID: 19096942
• The converging roles of Batten disease proteins in neurodegeneration and cancer.
  Yap SQ, Mathavarajah S, Huber RJ. Yap SQ, et al. iScience. 2021 Mar 19;24(4):102337. doi: 10.1016/j.isci.2021.102337. eCollection 2021 Apr 23. iScience. 2021. PMID: 33889828 Free PMC article. Review.
• Knock-down of cathepsin D affects the retinal pigment epithelium, impairs swim-bladder ontogenesis and causes premature death in zebrafish.
  Follo C, Ozzano M, Mugoni V, Castino R, Santoro M, Isidoro C. Follo C, et al. PLoS One. 2011;6(7):e21908. doi: 10.1371/journal.pone.0021908. Epub 2011 Jul 1. PLoS One. 2011. PMID: 21747967 Free PMC article.
• Quantitative proteomic analysis identifying three annexins as lymph node metastasis-related proteins in lung adenocarcinoma.
  Liu YF, Chen YH, Li MY, Zhang PF, Peng F, Li GQ, Xiao ZQ, Chen ZC. Liu YF, et al. Med Oncol. 2012 Mar;29(1):174-84. doi: 10.1007/s12032-010-9761-3. Epub 2010 Dec 4. Med Oncol. 2012. PMID: 21132403
• Quantitative proteome analysis of colorectal cancer-related differential proteins.
  Zhang Y, Liu Y, Ye Y, Shen D, Zhang H, Huang H, Li S, Wang S, Ren J. Zhang Y, et al. J Cancer Res Clin Oncol. 2017 Feb;143(2):233-241. doi: 10.1007/s00432-016-2274-5. Epub 2016 Sep 22. J Cancer Res Clin Oncol. 2017. PMID: 27659785

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • American Chemical Society